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An equal amount of protein was separated on 10%-12% SDS-PAGE gels and transferred onto polyvinylidene difluoride membrane (Pall). The nonspecific binding sites were blocked with TBST buffer [NaCl (150 mmol/L), Tris-HCl (20 mmol/L, pH7.4), and Tween 20 (0.4%, v/v)] containing nonfat dry milk (5%) for 2 h. The membranes were incubated overnight, at 4��C, with specific primary antibodies (at a 1:1000 dilution). Next, the membranes were washed three times with TBST buffer, and incubated at room temperature for 2 h with horseradish peroxidase-conjugated secondary antibody (at a 1:5000 dilution). Membranes were then washed Venetoclax nmr three times with TBST buffer. The immunoblots were visualized using an enhanced Phototope-Horseradish Peroxidase Detection Kit, purchased from Cell Signaling Technology, and film was developed using a Kodak medical X-ray processor. Statistical analysis Results were analyzed using MMP23B a t-test or one-way ANOVA, and SPSS 19.0 software. Data were presented as mean �� SD using a minimum of triplicate determinations. *, P find more on the 11th day after implantation. The various treatments were as follows: ... Table 1 Effect of HM910 on inhibiting multiple myeloma cell NCI-H929 growth in vivo HM910 induced cell cycle arrest in G1 phase in MM cells To explore the mode of action, NCI-H929 cells were exposed to HM910 for 24h and then cell cycle was analyzed. HM910 resulted in an increased number of cells in G1 phase (Figure 3). Furthermore, we observed that G1 phase related proteins, such as CDK4, CDK6, c-myc, and cyclin D1 expression levels decreased in the presence of HM910 (Figure 3C).