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Rabies virus was isolated from CSF and saliva, and rabies antibody was demonstrated in serum (from day 11 onwards) and cerebrospinal fluid (day 13 onwards). She died on day-35 of hospitalization. Autopsy specimens demonstrated the presence of rabies antigen, viral RNA, and viable rabies virus in the central nervous system. J. Med. Virol. 82:1255�C1265, 2010. ? 2010 Wiley-Liss, Inc. ""Objective?To assess the effects on physiology and behaviour of intradermal injection of sodium lauryl sulfate (SLS) as an alternative to mulesing. Procedures?Three groups of Merino lambs were studied: Control (n = 10), SLS (n = 11) and Mulesed (n = 11). The SLS group received SLS (7% w/v) and benzyl alcohol (20�mg/mL) in phosphate AZD8055 solubility dmso buffer, and the Mulesed group received 6�mL topical local anaesthetic as a wound dressing. Haematology, cortisol, beta-endorphin and haptoglobin concentrations, rectal temperatures, body weight and behaviours were monitored for up to 42 days post treatments. Results?SLS treatment induced mild swelling followed by thin scab formation. Fever (>40�C) was observed at 12 and 24�h, cortisol concentration was elevated on days 1 and 2, haptoglobin concentration was highly elevated on days 2�C7, white blood cell count was elevated on days 2 and 4 post treatment, but average daily gain was not affected. Fever at 12?h was significantly higher in the SLS than in the Mulesed group, Anti-cancer Compound Library screening whereas maximum temperature, temperature area under the curve (AUC), occurrence of fever, cortisol profile, cortisol AUC, white blood cell counts and haptoglobin concentrations until day 7 were comparable. The behaviours of normal standing, total standing and total lying were modified for 2 days by SLS treatment, but changes were less marked and of shorter duration than in the Mulesed group. On day 1, the SLS Ceramidase group spent