Top 7 Stuff You Don't Learn Concerning Nutlin-3
The beta diversity measures for the comparison of the rumen methanogen community structure showed a separation from the small ruminant and large ruminant groups explained by the first axis of variance (25.5%), while a smaller percentage of variance was observed from the cattle and yak samples along the second axis (16.1%) (Supplementary Figure??2). Figure 2 Rumen methanogen community heat maps and clustering of the most abundant 100 OTUs using regularized log transformed values from DeSeq2. Ward's INPP5D minimum variance method was used for hierarchical clustering of the computed distance matrix for samples based ... The most abundant OTUs in both of the crossbred sheep and Tibetan sheep were associated with the Mmc. group 10, while for cattle the most abundant OTU was associated with Mbr. gottschalkii Nutlin 3 and for the yak the most abundant one was an OTU that was identified as being affiliated with the Mbr. sp. RT. In all animals the two most abundant Mbr. gottschalkii OTUs were the same as was their rank order. More variance in the rank order was observed for the Methanomassiliicoccaceae OTUs. However, all animals were dominated by the same Mmc. group 4 and Mmc. group 10 OTUs. Changes in the most abundant OTUs affiliated with a Mmc. group 12 were observed for animals with cattle and yak having different population from that of the crossbred sheep and Tibetan sheep (Figure 2). Analysis of changes in the abundance of these OTUs for specific animal pairwise comparisons is presented in the Supplementary Figures and statistically confirms the observations shown in Figure 2 (Supplementary Figures??3�C8 for further details). Furthermore OTUs common to all animals as indicated in Figure 2 were not found to be significantly different in pairwise comparisons (Supplementary Figures??3�C8). The abundance of Methanomassiliicoccaceae members and Methanobrevibacter species RO4929097 ic50 as measured with qPCR is presented in Table 3 and is in agreement with the 16S sequencing data. Tibetan sheep had a significantly higher abundance (P