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Guidelines recommend the performance of HIV-1 genotypic resistance testing when managing suboptimal viral load reduction, and to assist in the selection of active drugs when changing antiretroviral regimens in patients with virological failure and HIV-1 RNA levels >1000?copies/mL LY2109761 cost [1,2]. In persons with >500 but the wide variability of skill in performing resistance testing among different laboratories. In real life, many reports have shown the highly successful use of genotypic resistance testing in HIV-1-infected patients with detectable viraemia between 50 and 1000?copies/mL [37�C39]. In addition, multiple drug resistance mutations can also be selected and detected at HIV-1 rebound with low viral loads [37,40]. Indeed, resistance mutations have been found to accumulate in 68�C93% of patients with persistent viraemia between 50 and 1000 copies/mL [41]. Therefore, in patients with a suboptimal response, resistance testing at week?4 is recommended. Genotype validity with plasma EPZ5676 in vitro HIV-1 RNA level Oxacillin of a virology working routine. The histograms represent the number of GRTs performed at the first failure, according to viral load and year of treatment start. Interestingly, the percentage of GRTs with viral loads