?169.7 ?.24 ?15.*Values are medians (25th, 75th percentiles) and comparisons were made by

{Cases: initial, n ?49; follow-up, n ?32. {Controls: initial, n ?39; follow-up, n ?17. �P values fpsyg.2015.01865 immunosorbent assay with recombinant p23 as antigen in children with cryptosporidiosis in Bangladesh. (Pb; N = 7), histoplasmosis (Hc; N = six), aspergillosis (Af; N = five), chromomycosis (Cr; N Changes in antibody levels over the three-week follow-up period were compared by using the unpaired t-test with Welch's correction.BORAD AND OTHERSthose to the immunodominant gp15 antigen, or to total antigens in C. S of secreted IL-1, IL6, IL-12p70, and TNF- by standard parvum lysate, we compared antibody levels to p23 with each of these antigen preparations from previous studies in the same children.43,46 There were significant correlations in the initial, follow-up, and change between the IgG, IgA, and IgM (except for IgM follow-up) antibody levels to p23 with those to gp15 in cases over the three-week follow-up period (Table 3). In addition, there were significant correlations in antibody levels (except for IgG follow-up and change) to p23 with those to C. parvum lysate at the initial, follow-up, and change between them (Table 3). Analysis of p23 polymorphisms among infecting jir.2011.0094 Cryptosporidium spp. subtypes. Molecular characterization of the Cryptosporidium spp. infecting case children in the study identified seven subtype families from C. parvum (IIm), C. hominis (Ia, Ib, Id, Ie and If), and C. felis (IIa).47 The C. parvum IIm subtype family has only recently been identified in Bangladesh,47 India,49 and Nigeria,51 and is considered an anthroponotic subtype because it has not been identified in animals. The other anthroponotic subtype family, IIc,41 was not identified in this study. We therefore amplified the p23 sequence from DNA from stool samples of three children infected with this subtype family in a previous study on cryptosporidiosis in children with diarrhea in Vellore, India.48 To determine if there were differences in the p23 sequences from subtype families of C. parvum and C. hominis spp. infecting children in this study with those in the published C. parvum52 and C. hominis53 genomes, as well as other p23 sequences deposited in GenBank, we performed phylogenetic analysis. This analysis showed that the p23 nucleotide and deduced amino acid sequences from the major species clustered separately for the most part (Figure 4).TABLE 3 Correlation of antibody levels to Cryptosporidium (C) p23 with antibody levels to gp15 and C. parvum lysate at initial and follow-up points and their change from initial to follow-up time points in children with cryptosporidiosis, Bangladesh*Characteristic Spearman correlation valuesCp23 vs. gp15 IgG Initial Follow-up Change IgA Initial Follow-up Change IgM Iintial Follow-up Change Cp23 vs. C. parvum lysate IgG Initial Follow-up Change IgA Initial Follow-up Change IgM Initial Follow-up Change*Cases: initial, n ?49; follow-up, n ?32; change, n ?32.?169.7 ?.24 ?15.*Values are medians (25th, 75th percentiles) and comparisons were made by using the Mann-Whitney test unless otherwise indicated.