1 vs. WT OXA) (Figure 5A and 5B).NIH-PA Author Manuscript NIH-PA

WT OXA) (Figure 5A and 5B).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptSTAT6 regulates expression of IL-33 and TSLP in oxazolone Compared with 23 for those not exposed to ETS.31 Race/ethnicity of colitis Oxazolone colitis was initially described as a Th2-driven model of colitis with increased lamina propria lymphocyte production of IL-4 and IL-13 (9, ten). Considering that STAT6 is an crucial regulator of Th2 lymphocyte differentiation (21), we hypothesized expression of IL-4 and IL-13 will be reduced or absent in STAT6-/- in comparison with WT OXA mice. Interestingly, applying real-time PCR evaluation of title= eLife.16793 colon tissue mRNA, we observed a mixed Thelper lymphocyte response with drastically elevated expression of IL-13, IFN-, IL-17, and IL-10 (IL-4 was not Lts have been summarized with respect to overall mobility prices and distance detected by real-time PCR, data not shown) in WT OXA compared to WT ETOH mice.1 vs. WT OXA) (Figure 5A and 5B).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptSTAT6 regulates expression of IL-33 and TSLP in oxazolone colitis Oxazolone colitis was initially described as a Th2-driven model of colitis with improved lamina propria lymphocyte production of IL-4 and IL-13 (9, 10). Due to the fact STAT6 is an vital regulator of Th2 lymphocyte differentiation (21), we hypothesized expression of IL-4 and IL-13 will be decreased or absent in STAT6-/- when compared with WT OXA mice. Interestingly, using real-time PCR analysis of title= eLife.16793 colon tissue mRNA, we observed a mixed Thelper lymphocyte response with considerably elevated expression of IL-13, IFN-, IL-17, and IL-10 (IL-4 was not detected by real-time PCR, information not shown) in WT OXA compared to WT ETOH mice. In addition, there was no detectable distinction in expression of these cytokines amongst WT OXA and STAT6-/- OXA mice (Figure 6A). In current years, IL-33, thymic stromal lymphopoietin (TSLP), and IL-25 have emerged as essential cytokines for the initiation and amplification of Th2 immune responses (24). We observed a five.6-fold elevated relative expression of IL-33 in WT OXA mice as in comparison with WT ETOH mice, which was abrogated in STAT6-/- OXA mice. We also observed a 2-fold increased relative expression of TSLP in WT OXA mice which was eliminated in STAT6-/- OXA mice (Figure 6A). We didn't observe any distinction in IL-25 expression amongst mouse groups (information not shown).1 vs. WT OXA) (Figure 5A and 5B).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptSTAT6 regulates expression of IL-33 and TSLP in oxazolone colitis Oxazolone colitis was initially described as a Th2-driven model of colitis with improved lamina propria lymphocyte production of IL-4 and IL-13 (9, 10). Considering that STAT6 is definitely an significant regulator of Th2 lymphocyte differentiation (21), we hypothesized expression of IL-4 and IL-13 would be lowered or absent in STAT6-/- compared to WT OXA mice. Interestingly, applying real-time PCR analysis of title= eLife.16793 colon tissue mRNA, we observed a mixed Thelper lymphocyte response with significantly increased expression of IL-13, IFN-, IL-17, and IL-10 (IL-4 was not detected by real-time PCR, data not shown) in WT OXA in comparison with WT ETOH mice.