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96) and all other probes was conducted. A gene was considered to have consistent correlation if it showed correlation of �� > 0.4 (positive) or �� selleck inhibitor averaged to give a single value per gene. Hierarchical clustering of the genes was performed using the Pearson correlation and complete linkage. Molecular class was predicted from the metagene signatures for the four classes. Analysis was performed using R Software. Results are represented as means �� SEM. Differences were analyzed by unpaired two-tailed Student's t test or one-way analysis of variance using Prism 5 (GraphPad Software). Thymidine kinase For Figures?4C and W1C, Dunn multiple-comparison test was used. ANCOVA was used for age-adjusted tests of means, and Cox regression was used for survival analysis (R Software). P BAY-61-3606 mw were analyzed. Sox2 expression obtained from two Affymetrix U133 probes were not significantly different between primary and recurrent GBMs (213721_at, P = .4618; 213722_at, P = .4293) ( Figure?1D). Sox2 expression tended to decrease on recurrence for the younger patients and for the grade III tumors ( Figure?1D). Together, these observations indicate that Sox2 expression is not restricted to a small subpopulation of undifferentiated neoplastic cells, suggesting that Sox2 could regulate different programs in CSCs and more differentiated GBM cells.