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Версія від 04:48, 19 червня 2017, створена Yarn43angle (обговореннявнесок) (Створена сторінка: Material and Methods:? Forty adult Albino rats were divided into four groups: (1)?control group; (2)?experimentally induced periodontitis group; (3)?experimenta...)

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Material and Methods:? Forty adult Albino rats were divided into four groups: (1)?control group; (2)?experimentally induced periodontitis group; (3)?experimentally Unoprostone induced osteoporosis group; and (4)?experimentally induced osteoporosis and periodontitis group. At the end of the experimental period, blood samples were obtained and animals were sacrificed. Serum alkaline phosphatase (ALP) activity levels were measured. Histological evaluation and immunohistochemical detection of RANKL in the periodontal ligament and bone tissues were performed. Results:? There were significantly higher ALP levels in all of the experimental groups than in the control group. The pathology observed in the histological sections from group?4 was more severe than in either group?2 or group?3. The percentage of RANKL-immunoreactive cells in both the periodontal ligament and bone tissues in group?4 (16.8?��?5.1 and 11.2?��?5.2%, respectively) was significantly higher (p?BGJ398 research buy qualitative assessment of morphologic changes within human gingival cells and tissues are still unexplored. The aim of this work is to assess the structural gingival changes in patients with generalized chronic periodontitis (GCP), before and after scaling and root planing (SRP) by using fractal and selleck screening library multifractal analysis. Twelve adults with untreated chronic periodontitis were treated only by SRP. At baseline and after SRP, gingivomucosal biopsies were collected for histopathological examination. Fractal and multifractal analysis of digital images of the granular, spinous and basal and conjunctive layers structure, using the standard box-counting method was performed. The fractal dimension was determined for cell membrane, nuclear membrane of cell and nucleolus membrane of cell. In GCP a higher fractal dimension corresponds to a higher geometric complexity of cells contour, as its values increase when the contour irregularities increase. The generalized fractal dimensions were determined for the conjunctive layer structure of patients with GCP and patients with GCP and SRP. The fractal and multifractal analysis of gingival biopsies confirmed earlier findings that SRP reduces gingival injury in patients with GCP.