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Grading for each set of 10 slides were done for both eosin [Table 1], turmeric [Table 2] and comparison between the staining ability of eosin BEZ235 and turmeric was made [Table 3]. Table 1 Grading of each set of (10) slides stained with eosin for various tissue structures by 10 independent observers Table 2 Grading of each set of (10) slides stained with turmeric for various tissue structures by 10 independent observers Table 3 Comparison of staining ability of eosin and turmeric for various tissue structures Statistical analysis revealed that, overall staining ability of eosin is better than turmeric. However, interestingly collagen, muscle fibers shows P BLU9931 dye stain these structures in a remarkably different and distinct pattern giving deep yellowish orange color when compared to other tissues as suggested by their P values, which were 0.143 and 0.280 respectively. Discussion Turmeric is a rhizomatous herb that belongs to the family Zingiberaceae and the major species is genus C. longa.[4] 17-DMAG (Alvespimycin) HCl It is a medicinal plant extensively used in Ayurveda, Unani and Siddha medicine as a home remedy for various diseases including biliary disorders, anorexia, cough, diabetic wounds, hepatic disorders, rheumatism, and sinusitis. Curcumin (Diferuloylmethane), the main yellow bioactive component of turmeric has been shown to have a wide spectrum of biological actions - anti-inflammatory, antioxidant, anticarcinogenic, antimutagenic, anticoagulant, antidiabetic, antibacterial, antifungal, antiprotozoal, antiviral, antifibrotic, antivenom, antiulcer, hypotensive and hypocholesteremic activities.[5] Since long turmeric or C. longa is valued for its principle coloring constituent curcumin, which imparts yellow color to textile fibers and food.[6] The ability of a dye to stain specific tissue structures is determined by certain factors, one of which is the acidity of the stain. Acidic structures would be stained by basic dyes while basic structures would be stained by acidic dyes.[3] Owing to the strong affinity of C.