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The level of significance was set at 5% for all tests. The size of the myocardial infarction area is shown in Fig.?2. There were no differences in the area of myocardial infarction induced by coronary artery ligation in both protocols, a critical condition MLN8237 cost for the analysis of the biochemical and echocardiographic parameters presented here. No differences were observed in the bodyweights of the rats throughout the experiments (repeated measures anova, P?= 0.662; Table?1). Figures?3 and 4 show assessment of cardiac function obtained in the two protocols (A and B, respectively). The left ventricle EF was lowered ~?37% in response to myocardial infarction (protocol A: C vs I, P?=?0.001; protocol B: C vs I, P?=?0.002). Atorvastatin treatment showed only a transitory find more beneficial effect that was observed in 48?h (I vs IAt, P?=?0.042), but not after 7?days. Similarly to EF, the myocardial infarction reduced the left ventricular shortening fraction in both protocol A (C vs I, P?Aldosterone Plasma cytokines (IL-6, TNF-�� and IL-10) were only measured in protocol A, and are shown in Fig.?5. Acute myocardial infarction induced an increase in plasma levels of both pro-inflammatory (IL-6 and TNF-��: C vs I, P?