Ces, 60 nitrogen sources, and 15 sulfur sources made use of as nutrients (Table S
Ces, 60 nitrogen sources, and 15 sulfur sources employed as nutrients (Table S2). In total 425 pathways for metabolism of different compounds were delineated. This analysis confirms the limited capability of P. putida to work with sugars as a C supply, which is restricted to glucose, gluconate and fructose. DOT-T1E features a comprehensive Entner oudoroff route for utilization of glucose and also other hexoses, but lacks the 6-phosphofructokinase of the?2013 The Authors. Microbial Biotechnology published by John Wiley Sons Ltd and Society for Applied Microbiology, Microbial Biotechnology, 6, 598?602 Z. Udaondo et al.Fig. 3. Distribution of enzyme activities of P. putida DOT-T1E classified according to the EC nomenclature. For complete facts with the EC classification the reader is Trochol web referred to http:// www.chem.qmul.ac.uk/iubmb/enzyme/.glycolytic pathway, in agreement using the genome analysis of others Pseudomonads (del Castillo et al., 2007). three. Distribution of enzyme activities of P. putida DOT-T1E classified in accordance with the EC nomenclature. (A) EC X; (B) EC XX; and (C) EC XXX. Colour code for classes and subclasses by numbers are indicated. For full specifics of the EC classification the reader is referred to http:// www.chem.qmul.ac.uk/iubmb/enzyme/.glycolytic pathway, in agreement with all the genome analysis of other individuals Pseudomonads (del Castillo et al., 2007). A big variety of sugars had been found to not be metabolized by T1E which includes xylulose, xylose, ribulose, lyxose, mannose, sorbose, D-mannose, alginate, rhamnose, rhamnofuranose, galactose, lactose, epimelibiose, raffinose, sucrose, stachyose, manninotriose, melibiose, tagatose, starch and cello-oligosaccharides, to cite some, in agreement using the lack of genes for the metabolism of those chemical compounds soon after the genome analysis of this strain. The outcomes also confirmed the capacity of P. putida to work with as a C source organic acids (for instance acetic, citric, glutaric, quinic, lactic and succinic amongst other individuals), certain L-amino acids (Ala, Arg, Asn, Glu, His, Ile, Lys, Pro, Tyr and Val),and several amino organic compounds. (See Figs S1 4 for examples of catabolic pathways for sugars, amino acids, organic acids and aromatic compounds catabolism.) Strain T1E harbours genes to get a restricted quantity of central pathways for metabolism of aromatic compounds and a lot of peripheral pathways for funnelling of aromatic compounds to these central pathways. As in other Pseudomonads certainly one of the techniques exploited by this microbe for the degradation of different aromatic compounds will be to modify their diverse structures to prevalent dihydroxylated intermediates (Dagley, 1971); one more strategy should be to produce acyl-CoA derivatives for instance phenylacetyl-CoA (Fern dez et al., 2006). Concerning?2013 The Authors. Microbial Biotechnology published by John Wiley Sons Ltd and Society for Applied Microbiology, Microbial Biotechnology, 6, 598?Solvent tolerance methods peripheral pathways the P. putida DOT-T1E genome evaluation has revealed determinants for putative enzymes in a position to transform several different aromatic compounds. The DOT-T1E strain is able to make use of aromatic hydrocarbons for example toluene, ethylbenzene, benzene and propylbenzene to cite some (Mosqueda et al., 1999). The strain also makes use of aromatic alcohols for example conyferyl- and coumaryl-alcohols and their aldehydes; a range of aromatic acids including ferulate, vanillate, p-coumarate, p-hydroxybenzoate, p-hydroxyphenylpyruvate, phenylpyruvate, salicylate, gallate and benzoate (see Fig.