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putida DOT-T1E Ings have been offered {directly|straight classified according to the EC nomenclature. (A) EC X; (B) EC XX; and (C) EC XXX. Colour code for classes and subclasses by numbers are indicated. For full details in the EC classification the reader is referred to http:// www.chem.qmul.ac.uk/iubmb/enzyme/.glycolytic pathway, in agreement with all the genome analysis of other individuals Pseudomonads (del Castillo et al., 2007). The results also confirmed the capacity of P. putida to work with as a C supply organic acids (like acetic, citric, glutaric, quinic, lactic and succinic among others), certain L-amino acids (Ala, Arg, Asn, Glu, His, Ile, Lys, Pro, Tyr and Val),and numerous amino organic compounds. (See Figs S1 four for examples of catabolic pathways for sugars, amino acids, organic acids and aromatic compounds catabolism.) Strain T1E harbours genes for any restricted quantity of central pathways for metabolism of aromatic compounds and numerous peripheral pathways for funnelling of aromatic compounds to these central pathways. As in other Pseudomonads among the strategies exploited by this microbe for the degradation of different aromatic compounds would be to modify their diverse structures to common dihydroxylated intermediates (Dagley, 1971); one more strategy will be to produce acyl-CoA derivatives like phenylacetyl-CoA (Fern dez et al., 2006). Regarding?2013 The Authors. Microbial Biotechnology published by John Wiley Sons Ltd and Society for Applied Microbiology, Microbial Biotechnology, 6, 598?Solvent tolerance methods peripheral pathways the P. putida DOT-T1E genome analysis has revealed determinants for putative enzymes in a position to transform various aromatic compounds. The DOT-T1E strain is able to use aromatic hydrocarbons which include toluene, ethylbenzene, benzene and propylbenzene to cite some (Mosqueda et al., 1999). The strain also makes use of aromatic alcohols like conyferyl- and coumaryl-alcohols and their aldehydes; a array of aromatic acids which include ferulate, vanillate, p-coumarate, p-hydroxybenzoate, p-hydroxyphenylpyruvate, phenylpyruvate, salicylate, gallate and benzoate (see Fig. S4).Ces, 60 nitrogen sources, and 15 sulfur sources employed as nutrients (Table S2). In total 425 pathways for metabolism of unique compounds were delineated. This analysis confirms the limited capacity of P. putida to utilize sugars as a C supply, which is restricted to glucose, gluconate and fructose. DOT-T1E features a full Entner oudoroff route for utilization of glucose along with other hexoses, but lacks the 6-phosphofructokinase on the?2013 The Authors. Microbial Biotechnology published by John Wiley Sons Ltd and Society for Applied Microbiology, Microbial Biotechnology, 6, 598?602 Z. Udaondo et al.Fig. 3. Distribution of enzyme activities of P. putida DOT-T1E classified as outlined by the EC nomenclature. (A) EC X; (B) EC XX; and (C) EC XXX. Colour code for classes and subclasses by numbers are indicated.Ces, 60 nitrogen sources, and 15 sulfur sources utilized as nutrients (Table S2). In total 425 pathways for metabolism of distinct compounds had been delineated. This analysis confirms the restricted ability of P. putida to use sugars as a C source, which is restricted to glucose, gluconate and fructose. DOT-T1E features a full Entner oudoroff route for utilization of glucose and also other hexoses, but lacks the 6-phosphofructokinase in the?2013 The Authors. Microbial Biotechnology published by John Wiley Sons Ltd and Society for Applied Microbiology, Microbial Biotechnology, six, 598?602 Z. Udaondo et al.Fig. three. Distribution of enzyme activities of P. putida DOT-T1E classified according to the EC nomenclature.