Childs, Work Then Megestrol Acetate

, 2010). To assess whether the lack of a small subset of key proteins from Pclag-cdpk1-gfp ookinetes was a consequence of changed transcription, we compared the transcriptomes of wild-type and mutant ookinetes by RNA sequencing. PD-0332991 research buy Two hundred fifty-two of 4,692 transcripts (5.5%) were significantly less abundant in the mutant, while 65 (1.3%) were more abundant (p?Selleckchem GSK2118436 the degree of dysregulation of transcript and protein against each other ( Figure?5A) and assigned all dysregulated genes to categories according to the direction and degree of their alteration ( Figure?5B). Only five genes showed evidence for significant coregulation of transcript and protein between wild-type and mutant (listed in Table S2). The single largest dysregulation category contained 65 genes, all of which had reduced protein levels despite normal mRNA counts ( Figure?5B and Table S2). This group was characterized by an 8-fold enrichment in genes whose mRNAs were shown previously to rely on either the DOZI helicase or CITH to be stabilized in gametocytes ( Mair et?al., 2006, 2010). It is thought that these transcripts associate with mRNP complexes defined by DOZI and CITH to achieve stability Megestrol Acetate and prevent early translation. The enrichment for suspected translationally repressed transcripts among the dysregulated proteins of the Pclag-cdpk1-gfp was highly significant (p?