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Cohort description For this sub-study, all of the patients within the EuSOS database were included. Patients lacking a description of their ASA status were excluded from the study (92 patients). Other exclusion criteria derived from the sensitivity analysis of the EuSOS score and defined to exclude the effects of very small centers or extreme deviations regarding the reported mortality were as follows: (1) any site that enrolled less than 10 patients during the study week, (2) any site with a hospital mortality rate either above the 95th centile or below the 5th centile, and (3) any patient with missing data for hospital mortality. Outcomes The primary outcome used in Nutlin-3 cell line this study was survival at the time of hospital discharge. Patients were followed until hospital discharge, death or 60 days after hospital admission. Statistical analysis The data were analyzed using SPSS, version 19.0 (SPSS Inc, Chicago, USA). Categorical variables are presented as numbers (%), and continuous variables are presented as means (SD) when normally distributed or medians (IQR) when not normally distributed. the Chi squared and Fisher��s exact tests were to compare categorical variables, and the t test or ANOVA was used to compare continuous variables. Significance was set at ABT 737 p Thymidine kinase hospital mortality was affected by other variables. To minimize the correlation with variables that were already included in the ASA score, comorbid diseases that were present at hospital admission were not used in the model because they are included in the definitions of the first 3 classes of the ASA score. The first step was to identify factors that were independently related to hospital mortality in the multivariate analysis. The following factors were entered into the model based on their relationship to the outcome in the univariate analysis: age, gender, urgency of surgery (reference urgent), laparoscopic surgery, seniority of the surgeon, seniority of the anesthesiologist, grade of surgery and surgical procedure category. Due to the multiplicity of tests performed and to avoid spurious associations and over-fitting, only p values less than 0.