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Версія від 19:48, 18 червня 2017, створена Iranchild1 (обговореннявнесок) (Створена сторінка: Lastly, we show that the co-expression of Ato with [http://www.selleckchem.com/products/BMS-777607.html BMS777607] the Senseless (Sens) zinc finger protein that...)

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Lastly, we show that the co-expression of Ato with BMS777607 the Senseless (Sens) zinc finger protein that is essential for nearly all peripheral nervous system development also promotes the formation of many ch organ SOP cells ( Nolo et al., 2000). Importantly, Ato and Sens induce significantly more ch organ SOP cells in the abdomen than in the thorax, and surprisingly do so using both Spi-dependent and Spi-independent mechanisms. In total, these experiments reveal that genetic interactions between the EGF pathway, the Abd-A Hox factor, and the Sens zinc finger protein enhance the sensory organ promoting activity of Ato to result in region-specific neurogenesis in the Drosophila abdomen. Expression of the Rho protease within a set of abdominal ch organ SOP cells triggers the secretion of the Spi selleck inhibitor EGF ligand to recruit abdomen-specific oenocytes and 2�� ch organ SOP cells (Fig.?1). We recently identified a rho enhancer (RhoBAD) that is expressed within the same abdominal C1 SOP cells that induce oenocyte formation ( Fig.?2A) and found that both RhoBAD activity and oenocyte recruitment are dependent upon the Ato proneural factor ( Gebelein, 2008, Li-Kroeger et al., 2008?and?Witt et al., 2010). Moreover, the ectopic expression of Ato in every other segment of the developing embryo using the PrdG4 driver results in the stimulation of RhoBAD-lacZ activity in additional cells ( Fig.?2B). These findings indicate that ectopic Ato activates additional rho expression, which should subsequently enhance Spi secretion to induce more oenocytes. However, expression Evodiamine analysis of PrdG4;UAS-Ato embryos for an early oenocyte marker (high levels of Salm in cells surrounding C1 SOP cells) revealed a sharp decrease in oenocytes within the PrdG4-driven Ato-expressing segments ( Fig.?2B). Analysis of older embryos using an additional marker of oenocyte fate (HNF4, ( Palanker et al., 2009)) confirmed this finding, with most Ato-expressing segments showing a complete loss of oenocytes ( Fig.?2C and D). Quantification of the number of oenocytes that develop within PrdG4;UAS-Ato embryos revealed that only 0.8?��?0.2 oenocytes formed in the PrdG4-on Ato-expressing segments while 6.4?��?0.3 oenocytes formed in the PrdG4-off non-Ato expressing segments ( Fig.?2H, p-value?