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But during these years, I also watched Maribel grow as a person. From the somewhat revolutionary phase in her youth in the last days of Franco's dictatorship, when she was on the verge of going to the barricades to defend liberty, justice, and women's rights, she evolved into a mature and centred woman, happily married to our colleague Dr Jos�� Larracoechea and with two adored children, Mar��a and Juan. Bleomycin research buy But this transformation did not entail the loss of any of her outstanding characteristics, nor did she betray a single one of her youthful ideals. She was unable to tolerate injustice or nepotism, continued to rise up against the oppression of women, and refused to turn a blind eye to mediocrity or incompetence among professionals. I have known few people with such a firm sense of personal dignity, so loath to engage in deception, or with such a pure concept of friendship. For all of these reasons, so many of us will mourn her absence and cherish her memory forever. ""Coxiella burnetii is a small, polymorphic Gram-negative bacterium from 0.3 to 0.5 microns in size, belonging to the order Legionellales, family Coxiellaceae, and genus Coxiella ( Garrity et al., 2005). Important sources of human infection of Verteporfin research buy C. burnetii are small ruminants, such as sheep and goats, dogs, cats, guinea pigs and gerbils ( Bielawska-Dr��zd et al., 2013). According to recent studies, C. burnetii shows great phylogenetic similarity to bacteria from the order Legionellales. C. burnetii occurs in two different morphological forms, LCV (large cell variant) and SCV (small cell variant) ( Mallavia and Minnick, 1995). C. burnetii SCV is characterized by unique resistance to physical factors, such as ultraviolet radiation, and chemical factors. In Poland, several Q fever outbreaks were reported during last 60 years in Owczary, formerly Rzesz��w Mephenoxalone Province in 1956, Lesser Poland and Silesia Provinces in 2005, and Subcarpathian and Lublin Province in 2008. The largest Q fever outbreak occurred in 1982 in Ulh��wek, formerly Zamo?? Province, and resulted in 220 dairy cow cases and 1300 human cases, which was the largest Q fever outbreak in the human population of Poland (Bielawska-Dr��zd et al., 2013). According to Polish legislation, Q fever is not a disease that is mandatory for control and prevention (isap.sejm.gov.pl, Dz.U. z 2004, r. Nr 69, poz. 625). Despite the availability of Coxevac, vaccinations against Q fever in livestock are not routinely implemented. For detailed molecular characterization of the agent, phylogenetic method such as MST (multi spacer typing) was used (Glazunova et al., 2005?and?Li et al., 2009). The objective of this study was to examine environmental samples for the presence of C. burnetii using real-time PCR and genetic characterization of isolated strains using the MST method. Subsequently, the presence of C.