Modifications at the C-terminus made during synthesis consisted of an amide group (no tag) or an extension of the peptide by addition of -biotinyl-lysine-amide for the binding assays

H2O2 generation and migration capability measured with assembled devices were interpreted with standard Boyden transwell assays and the results confirmed that the fully assembled bi-module device could indeed monitor Answers of the peptide in water have been neutralized and passed via a one x 10 cm column of DEAE-Sephadex A-twenty five, Cl- kind, at pH five to six to eliminate trifluoroacetic acid. For in vivo scientific studies, svH1C was prepared by absorption of 1 g peptide on a two.5 X ten cm column of CM-Sephadex C-twenty five in 100 mM NaCl. The column was washed with two hundred mM NaCl and svH1C was then eluted with 500 mM NaCl that was well prepared in endotoxin-free of charge water (