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5:0.5). Both rutin and quercetin were detected under UV 254 and UV 366 nm before and after sprayed. AlCl3 spray reagent was used to detect flavonoid and the determination of the compounds amount was perform by densitometer. Experimental design Thirty two male Wistar rats aged 1.5-2 months were aclimatized for 1 week and were divided into 8 groups. Diabetic condition was induced by intraperitoneal injection of alloxan monohydrate solution (150 mg/kgBB) in rats that had fasted for 8 hours. Blood glucose levels were measured 72 hours after injection of alloxan. Rats with serum glucose levels �� 150 mg/dL is stated to be diabetes and used for further research. Treatments were conducted for 15 days, 2 times a day, with treatment groups as follows: normal group (no treatment), negative control (CMC-Na 0.5%), glibenclamide 0.45 mg/kgBW, ethanolic extract of A. indica 200 mg/kgBW, ethanolic extract of G. procumbens 150 mg/kgBW, combination of A. indica 150 mg/kgBW and PD173074 supplier G. procumbens 37.5 mg/kgBW (combination 1), combination of A. indica 100 mg/kgBW and G. procumbens 75 mg/kgBW (comb. 2), and combination of A. indica 50 mg/kgBW and G. procumbens 112.5 mg/kgBW (comb. 3). Preprandial and postprandial blood samples were collected on day Temsirolimus nmr 0, 5, 10, and 15. Blood glucose levels were determined by Glucose Oxidase Phenol Aminoantipyrine (GOD-PAP) method. Pancreas histological observation Pancreas histological observation was conducted on day 15, the end of the experimental period. Rats were euthanized by cervical dislocation and the pancreas were collected and fixed with 4% paraformaldehyde in phosphate buffer saline for 24 hours. The pancreas was then stained with Hematoxylin eosin (HE) for observation of islet Langerhans morphology. Pancreatic insulin expression was conducted by Imunohistochemistry (IHC) analysis. Statistical analysis Statistically significant difference of hypoglycemic effect by ANOVA continued with LSD. Significance was MRIP set at p