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4. Statistical analysis All statistical analyses were performed using the PASW ver. 18 (SPSS Inc., Chicago, IL, USA). The SR was grouped according to the severity of LSCS by performing quartile analysis with the SR 0.75Resminostat of these domains and their categories were determined. Inverse-variance weighting was selected Staurosporine molecular weight as it is typically used in statistical meta-analysis to combine the results from independent measurements and it is an appropriate method for creating a score. The score for all of the cases was obtained based on their weight by summing up the points of the four variables. Finally, the quartile analysis was used and a new classification for LSCS was proposed. For instance, for the SR, we first assigned a score of 1, 2, 3, and 4 based on the severity of LSCS in each case. Then, we added these scores for the whole sample size (n=357) to calculate the mean score and standard deviation. Later, the standard deviation was powered by 2 to estimate the variance. The weight for SR was derived from the Dorsomorphin mw above formula, and therefore, to calculate the weighted score for each case the initial score for each case multiplied it. The procedure was repeated for each parameter, and the addition of these weighted scores yielded the final score for each case ranging from 4 to 16.5. 5. Assessment of the intra- and interobserver reliability of the classification grading In order to validate the classification, 83 patients with all types of disorders were randomly selected. Two independent observers classified each case twice within a 2-week interval to measure the intra- and inter-observer differences. The weighted Kappa coefficient was calculated for each spine surgeon based on his first and second data report and comparison of kappa between spine surgeons. Kappa values ranged between 0 and 1; the greater the kappa value, the higher the concordance rate. Kappa value of 0 to 0.20 indicated slight agreement, 0.21 to 0.40 indicated fair agreement, 0.41 to 0.60 indicated moderate agreement, 0.61 to 0.80 indicated substantial perfect agreement, and ��0.81 was regarded as almost perfect agreement according to the interpretation by Landis and Koch [13]. 6.