S the complement of genes for utilization of urea either by way of
Seven genes involved in resistance to arsenite rsenate ntimonite efflux had been annotated. 4 of them arsHCBR made an operon (T1E_2719?2722), plus the 3 other genes associated to arsenite resistance (T1E_4939, T1E_4996 and T1E_1144) are scattered throughout the genome. Finally a single chromate resistance protein ChrA (T1E_3354) was located within the genome of T1E suggesting it's the responsible for chromate efflux in this strain. Biotransformation potential As mentioned above DOT-T1E has the potential to thrive in the presence of toxic organic solvents that usually type a biphasic system with water. This property may be exploited to develop double-phase biotransformation systems (organic solvent and water) in which water insoluble chemicals, toxic substrates or chemical products are kept within the organic phase. The main benefits of those systems are that the product(s) is(are) Blem becomes so much diffused as to affect most elements of continuously removed by a solvent phase, their toxic effects are decreased as well as the lifespan from the biocatalytic system is longer. Additionally, when the concentration from the product increases inside the organic phase, solution recovery is less difficult and much less expensive (Bruce and Daugulis, 1991; Leon et al., 1998). Rojas and colleagues (2004) demonstrated that P.S the complement of genes for utilization of urea either by means of direct conversion to ammonia (T1E_4304 through T1E_4306, ureABC) or by way of conversion 1st to urea-1-carboxylate (T1E_3118 via and 3809) and after that conversion to ammonia (T1E_3119 and T1E_3808) (Fig. 4). Information for the utilization of D- and L-amino acids as N sources had been published by Daniels and colleagues (2010). It was located that the wild-type DOT-T1E strain was capable to work with many either D- or L-amino acids (i.e. Based around the strain's genome sequence, 64 genes had been identified that encode proteins putatively involved in heavy metal resistance and homeostasis (Table 1). The majority with the P. putida T1E heavy metal resistance genes are identified spread all through the genome, and they're conserved among all sequenced P. putida strains. Up to three diverse systems potentially involved in simultaneous cobalt, zinc and cadmium resistance were identified. One of several cation efflux systems could be the CzcD (T1E_2808) immersed inside a cluster with the corresponding response regulator CzcR (T1E_2811) as well as the sensor histidine kinase encoded by the czcS gene (T1E_2812). An additional household of transporters that may well mediate the extrusion of those 3 heavy metal ions will be the a single encoded by the cadA1 (T1E_2820) and cadA2 (T1E_4489) genes; at the same time as by the resistance-nodulation-cell division (RND) pump CzcABC (T1E_5270, T1E_5271, T1E_ 5272). The CusABC efflux system (T1E_4694, T1E_ 4695, T1E_4696) is involved resistance to silver and copper ions. Seven genes involved in resistance to arsenite rsenate ntimonite efflux have been annotated. 4 of them arsHCBR produced an operon (T1E_2719?2722), and also the 3 other genes related to arsenite resistance (T1E_4939, T1E_4996 and T1E_1144) are scattered throughout the genome. Ultimately 1 chromate resistance protein ChrA (T1E_3354) was identified within the genome of T1E suggesting it really is the accountable for chromate efflux within this strain. Biotransformation prospective As pointed out above DOT-T1E has the potential to thrive inside the presence of toxic organic solvents that usually kind a biphasic system with water.