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Thus, the 11 patients were confirmed as hMPV infection and the sensitivity of rapid antigen test was 55 %. There was no difference between rapid antigen test positive and negative cases in their median age (92.5 vs 91; NS) and prevalence of pneumonia (83% vs 80%; NS). The average days from the onset of symptoms to sample collection was significantly shorter in rapid antigen test positive patients than that in negative patients (4.8 vs 11.2 days; p?ALK the rapid test within 4 days from the onset of symptoms at longest. Our data suggests the sensitivity of rapid antigen test is decreased if sample collection is delayed from the onset of symptoms. XU-GUANG GUO1,2,3, YONG XIA1,2,3, SHAN ZHOU4, JIA-YUN LIU4, YUE-YUN MA4 1Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, People's Republic of China, 2Department of Internal Medicine, The Third Clinical College of Guangzhou Medical University, Guangzhou, Guangdong, People's Republic of China, 3Center for Severe Maternal Treatment of Guangzhou City, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, People's Pazopanib nmr Republic of China, 4Center for Clinical Laboratory Medicine of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an, People's Republic of China Background and Aim of Study:?Streptococcus pneumoniae was recognized as a major cause of pneumonia, and is issued as part of many humoral immunity studies. Diagnosis is generally built on clinical suspicion along with a positive culture from a sample from virtually any place in the body. But the testing time is extremely long. This study aims to develop a rapid diagnostic method to identification of Streptococcus pneumoniae. Methods:?Our laboratory has in recent years Regorafenib developed a new platform called RealAmp, which combines loop-mediated isothermal amplification (LAMP) with a portable tube scanner real-time isothermal instrument for the rapid detection of Streptococcus pneumonia. Two pairs of amplification primers required for this method were the result of a conserved DNA sequence unique to the Streptococcus pneumoniae. The amplification was performed at 63 degree Celsius using SYBR Green for 60 minutes with the tube scanner set to collect fluorescence signals. Clinical samples of Streptococcus pneumoniae and other bacteria were applied in the preparation of the sensitivity and specificity of the primers by comparing with traditional culture method.