The Beneficial, The Not So Good Along with Dabrafenib

98, pbinedaline decreased cell viability, while MEL exerted no protective effect on diquat induced cytotoxicity. Figure 2 Viability of HeLa cells treated with different doses of melatonin (MEL) in the presence or absence of 100 ��M diquat (DIQ) for 24 h. Cell viability was evaluated by MTT test. Data are presented as mean �� SEM (n= 3�C16 per group). ... In studies of oxidative stress, ROS production was first analyzed in HeLa cells exposed to different doses of diquat in the presence or absence of melatonin (100 ��M) for 17 h. We found a significant effect of an interaction between diquat and melatonin (F (3,12)=12.90, phttp://www.selleckchem.com/products/bmn-673.html a dose- dependent manner, showing pro-oxidative effect of the highest (100 ��M) and the medium diquat dose (10 ��M). The lowest diquat dose (1 ��M) did not affect ROS production as compared to the control. The high dose of melatonin (100 ��M) did not influence endogenous ROS production in the absence of diquat but it enhanced the pro-oxidative effect of diquat in combination with all three diquat doses. Figure 3 The effect of diquat (DIQ) and melatonin (MEL) on the production of reactive oxygen species (ROS) in HeLa cells. ROS production is proportional to DCF fluorescence. Bars represent mean (�� SEM) of 3 Dabrafenib mouse trials. (A) Fold increase of DCF fluorescence ... In the second part of the ROS production study, HeLa cells were exposed to different doses of melatonin in the presence of the medium dose of diquat (10 ��M) for 17 h. We found significant differences between treatment groups (F (6,12)=4.04, p