Un-Answered Questions Towards Fossariinae Showcased
The use of TCS-containing toothpaste did not appear to lead to an increase in MIC of TCS of oral bacterial isolates. ""The objective of this study was to elucidate the effects of different growth factors on the migration of dental follicle cells (DFCs). DFCs are ectomesenchymally derived easily accessible Fossariinae multipotent stem cells. Cell migration is a crucial step in many biological processes but also for tissue engineering. Growth factors such as epidermal growth factor (EGF), bone morphogenetic protein-2 (BMP2) or transforming growth factor ��1 (TGF-��1) can be used to modify the behavior of cells. We used different migration assays (gel spot assay, scratch assay, transwell assay) to evaluate the influence of EGF, BMP2 and TGF-��1 on the migration of DFCs. We investigated the expression of migration-related genes after growth factor stimulation using the PCR array human cell motility. DFCs treated with BMP2 or TGF-��1 migrated faster than DFCs treated with EGF. Additionally, more migration-related genes are regulated after treatment with BMP2 or TGF-��1 than with EGF. TGF-��1 additionally functions as a chemoattractant for DFCs. Osteogenic differentiation markers were regulated after BMP2 treatment only. Whereas the strong migration induced by BMP2 was accompanied by beginning osteogenic differentiation buy PI3K Inhibitor Library the strong migration induced by TGF-��1 was directional. EGF exhibited not only the weakest migration stimulation but also the weakest induction of differentiation into mineralizing cells. ""Park J-C, So S-S, Jung I-H, Yun J-H, Choi S-H, Cho K-S, Kim C-S. Induction of bone formation by Escherichia coli-expressed recombinant human bone morphogenetic protein-2 using block-type macroporous biphasic calcium phosphate in orthotopic and ectopic rat models. J Periodont Res 2011; 46: 682�C690. ? 2011 John Sunitinib nmr Wiley & Sons A/S Background and Objective:? The potential of the Escherichia coli-expressed recombinant human bone morphogenetic protein-2 (ErhBMP-2) to support new bone formation/maturation using a block-type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model. Material and Methods:? Critical-size (��?8?mm) calvarial defects and subcutaneous pockets in 32 Sprague�CDawley rats received implants of rhBMP-2 (2.5?��g) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2- and 8-wk healing intervals (eight animals/group/interval). Results:? ErhBMP-2/bMBCP supported significantly greater bone formation at 2 and 8?wk (10.8% and 25.4%, respectively) than the control at 2 and 8?wk (5.3% and 14.0%, respectively) in calvarial defects (p?
