Перегляд вихідного коду сторінки Unveiled: Why VAV2 Tends To Make All Of Us More Happy

Third, in the lmg, amg and outer chiasmal glia (xgo) in the lamina region (Figs. 3D, D��, 4C) and inner chiasmal glia (xgi) between [http://www.selleckchem.com/products/lonafarnib-sch66336.html Lonafarnib] the medulla and lobula neuropil (Figs. 3E, E��, 4C), EcR-B1 was weakly expressed at 24?h APF. EcR-B1 expression was gone in the xgi at 36?h APF and in the lmg, amg, xgo at 48?h APF (Fig. 3F, F��). Finally, in the distal satellite glia (dsg) (Fig. 3C, C���), pseudocartridge glia (psug), carpet glia (cg) and neuropil glia (ng), EcR-B1 was not expressed at any stages (Fig. 4A�CD, Table S3). In the optic lobe, EcR-A and EcR-B1 were expressed in different patterns. This difference indicated that these isoforms had different roles in the optic lobe development. Therefore, we examined the roles of EcR-A and EcR-B1 in the optic lobe cell death using isoform-specific mutants. EcRQ50st and EcRW53st are EcR-B1 mutant alleles; each carries a stop codon within the EcR-B1-specific exon 2 ( Bender et al., 1997). The average number of dying cells in the optic lobe at 0?h APF in the trans-heterozygote (EcRQ50st/EcRW53st) was 509.5, which is nearly the same as 545.8, the number in the wild-type ( Fig. 5A). The average number of dying cells in the mutant at 12?h APF (690.6) was smaller, but not significantly different from that in the wild-type (854.4). In most mutant samples, the number of dying cells was about 800 at this stage, nearly the same as that in the wild type (4 of the 5 optic lobes, 4/5), while it was much smaller (about 300) in 1 of the 5 mutant samples. [http://en.wikipedia.org/wiki/VAV2 VAV2] At 24?h APF, the number of dying cells in the mutant decreased and was 67% smaller than that in the wild-type (363.8 (mutant) versus 1112.6 (wild type); P[http://www.selleckchem.com/screening/tyrosine-kinase-inhibitor-library.html Tyrosine Kinase Inhibitor Library] of dying cells in the mutant remained constant (about 300) from 48 to 72?h APF, during this period only a few cell deaths occur in the wild type. Taken together, these results indicated that EcR-B1 was not required for cell death at 0?h APF, but was required between 24 and 36?h APF. The temporal pattern of the number of dying cells in the optic lobe of EcR-A mutants was different from that of EcR-B1 mutants. EcR112 and EcR139 are EcR-A mutant alleles that carrying deficiencies of the EcR-A transcription start site and the EcR-A specific exon, respectively ( Carney et al., 2004). The average number of dying cells in the trans-heterozygote (EcR112/EcR139) at 0?h APF was 466, which was not significantly different from the 545.8 in the wild-type ( Fig. 5A). At 12?h APF, however, the average number of dying cells in the mutant increased dramatically to 1186.4, which was 39% and significantly larger than the 854.4 observed in the wild-type (P