What You Should Expect From the Aldosterone?

""A rapid method using liquid chromatography tandem mass spectrometry (LC-MS/MS) for the quantification of olanzapine (OLZ) in human plasma was developed and validated. Venlanfaxine was used as the internal standard (I.S.) and the samples were extracted from 400 ��L human plasma using methyl tert-butyl ether for liquid-liquid extraction. Chromatography was performed using an ACE C18, 125 mm �� 4.6 mm i.d., 5 ��m column. The mobile phase consisted of water with 0.1% formic acid for solvent A and acetonitrile with 0.1% formic acid for solvent B (50:50 v/v) in isocratic mode. The flow rate was 1.2 mL��min?1. The retention times for OLZ and the I.S. were 0.78 and 1.04 min, respectively. Tandem mass spectrometry operating in positive electrospray ionization mode with multiple reaction monitoring was used to detect OLZ and the I.S.: Aldosterone m/z selleckchem 313.1 > 256.1 and 278.1 > 260.2, respectively. No significant matrix effects were observed on OLZ and the I.S. retention times, and the mean recovery of OLZ was 90.08%. The assay was linear in the concentration range of 1�C20 ng��mL?1 (R2 = 0.9976). The intra- and inter-day precision were C59 nmr nitroglycerine. Responses to static hand-grip exercise (SHG), graded lower body negative pressure (LBNP, from ?20 to ?45 mmHg) and 45 deg HUT were evaluated on separate days. Bland�CAltman analysis indicated that SVMF yielded lower estimates than SVDU during infusion of 0.01 ��g kg?1 min?1 isoprenaline (SVMF 92.7 �� 15.5 versus SVDU 104.3 �� 22.9 ml, P= 0.03) and SHG (SVMF 78.8 �� 12.0 versus SVDU 106.1 �� 28.5 ml, P