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- Cells were being lysed in RIPA buffer, and proteins were being isolated from mobile lysates by immunoprecipitation as explained earlier
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- Cells were stimulated with 10 ng/ml hTNF-a with or without twenty five mg/ml crude extract or still left untreated as damaging control and stained with mouse anti-human ICAM-one mAB
- Cells were then treated with or devoid of PEITC
