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- The correlation between the survivin 231CC genotype and increased cancer risk has been identified by several other studies
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- The corresponding trans isomer is inactive increasing the heterocycle reactivity or could be alone a pharmacophore
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- The created knowledge was validated by Northern blot evaluation employing a picked number of genes for all probes and clones analyzed
- The crimson-fluorescent Taspase1 variants (Tasp-mCherry, prey) on the other hand Leukemic (K562) and solid tumor cells ended up transfected with the indicated amounts of the various indicator plasmids
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- The current examine also contributes to extend the expertise on molecular mechanisms that underlie mesenchymal mobile dysfunction
- The current reports concentrated exclusively on younger offspring to verify
- The current review aims to evaluate the mitochondrial dysfunction in angiotensin II induced hypertrophy in H9c2 cells and the protective results of B. diffusa against mitochondrial injury in cardiac hypertrophy
- The cytotoxic effect of Huaier extract combined with 3-MA and CQ treatment was measured by MTT
- The data show that the control gene expression used to define altered gene expression in either condition is comparable
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- The dcuS-mvenus fusion was built and inserted in the chromosome of E. coli as described in the Supplemental Info the gene fusion last but not least encodes DcuS(1-543)-(GH)-mVenus(1-240)
- The deacetylase Sirt1 is a adverse regulator of p53 activation and Necdin interactions with this protein potentiate
- The deacetylase Sirt1 is a unfavorable regulator of p53 activation and Necdin interactions with this protein potentiate
- The degenerative process can cause radiating pain and loss of mobility that have a major impact on the quality of life
- The degree of Wee1 mRNA was decreased by eighty % after 3 days of RNAi induction (Figure 2A, inset)
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- The density of whole bands, which includes the higher-molecular-mass and the reduced-molecular-mass bands ended up quantified together
- The depth of SIV penetration could be visualized by double immunofluorescence staining of the Muc5AC and SIV nucleoprotein
- The design was vitality-minimized and subjected to a molecular dynamics simulation utilizing drive fields construction of human transketolase
- The determined immune-associated genes give an priceless source for elucidating the mechanisms fundamental
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- The difference in action is in agreement with the number of attributes lined by every compound
- The difference in fiber variety alterations in D257A mice and regular ageing could also be interpreted as proof that mtDNA mutations are not the initiating occasion in fiber atrophy and sarcopenia in individuals
- The difference of required sample sizes was less pronounced when the power analysis was conducted with the data from the 10replicate experiment conducted during the short-day period of the year
- The differences in RER values in between lean and overweight dam offspring were greater suggesting impaired metabolic versatility
- The differences in the distributions of age, menstrual history, BMI, and family history of cancer between cases and cancer-free controls
- The differences of 2D seed protein profiles of Arabidopsis between wild types and several transformed plants were small and fell in the range of the differences among 12 Arabidopsis ecotypes
- The differential expression of the picked genes was deemed to be validated as the qPCR expression profile above the time training course of infestation for the vast majority of these genes mirrored that of the microarray info
- The differentially expressed genes identified by ESTs had been functionally categorized by querying the NCBI eukaryotic Orthologous Team (KOG) databases
- The digestion was performed at room temperature with gentle shaking, and the progress of the reaction was monitored by SDS-PAGE
- The dimer interface was evaluated via molecular dynamics simulations calculating the conversation energies with dimer balance
- The disrupted sleep sample likely demonstrates the aversive effects metabolism need to be regarded as also in this context
- The dissociations constants for NADH and NAD are in excellent agreement with the binding August Conformational Transform in OcDH The outcomes on the NMR-spectroscopic investigations not only recommend a clear order and seuqnece of substrate binding
- The dissociations constants for NADH and NAD are in fantastic agreement with the binding August Conformational Change in OcDH The results with the NMR-spectroscopic investigations not just recommend a clear order and seuqnece of substrate binding
- The dissociations constants for NADH and NAD are in good agreement together with the binding August Conformational Modify in OcDH The results in the NMR-spectroscopic investigations not just suggest a clear order and seuqnece of substrate binding
- The dissociations constants for NADH and NAD are in good agreement using the binding August Conformational Modify in OcDH The outcomes with the NMR-spectroscopic investigations not only suggest a clear order and seuqnece of substrate binding
- The dissociations constants for NADH and NAD are in great agreement with all the binding August Conformational Change in OcDH The results on the NMR-spectroscopic investigations not merely suggest a clear order and seuqnece of substrate binding
- The dissociations constants for NADH and NAD are in superior agreement using the binding August Conformational Transform in OcDH The outcomes in the NMR-spectroscopic investigations not only suggest a clear order and seuqnece of substrate binding
- The dissociations constants for NADH and NAD are in very good agreement together with the binding August Conformational Transform in OcDH The results on the NMR-spectroscopic investigations not just suggest a clear order and seuqnece of substrate binding
- The dissociations constants for NADH and NAD are in very good agreement using the binding August Conformational Adjust in OcDH The outcomes of your NMR-spectroscopic investigations not merely suggest a clear order and seuqnece of substrate binding
- The dissociations constants for NADH and NAD are in very good agreement with the binding August Conformational Alter in OcDH The outcomes from the NMR-spectroscopic investigations not merely suggest a clear order and seuqnece of substrate binding
- The distribution of novel members of a gene family in populations is strongly motivated by variety force
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- The ear thickness was measured before and 24 hr after bacterial injection, and was normalized to that of the PBS-injected controls
- The easy substitution of the para-hydroxy group on curcumin with a methoxy substitution enhanced inhibitor operate
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- The effect of FgZRA1 deletion on ZEN production could not be explained, its role in fungicide sensitivity and virulence was not investigated
- The effect of extract RA and antiviral compounds on viral penetration was determined by a modified plaque reduction assay
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- The effectiveness of systemic treatment method to suppress mind FAS action is nevertheless questionable in vitro
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- The efficacy of this combination was also confirmed in wild-variety p53 tumor xenograft types
- The efficacy of this mix was also confirmed in wild-sort p53 tumor xenograft versions
- The efficacy of this mixture was also confirmed in wild-sort p53 tumor xenograft designs
- The efficiency of systemic remedy to suppress brain FAS activity is however questionable in vitro
- The efficiency of systemic therapy to suppress mind FAS action is however questionable in vitro
- The efficiency of the array hybridizations was further assessed via scatter plots (knowledge not demonstrated) evaluating every array with every single other created
- The efficient focus of cilostazol was determined by in vitro and in vivo experiments
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- The eluted proteins have been separated by SDS-Webpage and detected utilizing an anti-MBP monoclonal antibody (HRP-conjugated) (New England BioLabs)
- The elution was repeated once, and the fractions were pooled. DTT was added to 5 mM, and the mixture was incubated at 37uC for 45 min
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- The endogenous protein was sensitive to the very same inhibitors as the purified protein
- The energy of this initial structure was minimized with 3,000 steps of conjugated gradient minimization using the CHARMM27 force field
- The energy required for the synthesis of PPi from Pi is lower than that required for the synthesis of ATP from ADP
- The enhance in Necdin expression in reaction to PyLT expression was not brought on by inactivation was dependent
- The enhanced phosphorylation of Mpk1 in cdc48-3 at high temperature suggests that the heat stress may be exacerbated in cdc48-3 mutant
- The ensuing fusion DNA fragments of DAFS-HRP-DAFGPI and Thy1S-HRPThy1GPI were independently subcloned into the EcoRV web site of pENTR1A no ccdB (Addgene quantity 17398)
- The ensuing viral particles were being concentrated by ultracentrifugation and the viral transductions were executed in hESCs media
- The entire SPM course of action. A commercial platform and an environment produced
- The entire names of the individual lipid species can be found in the text
- The enzyme action was decided 1 moment right after the response was started out
- The epidural catheter was inserted amongst the L2 and L4 interspaces in this review, which were not found in the middle of the incision segments
- The epidural catheter was inserted between the L2 and L4 interspaces in this research, which have been not positioned in the middle of the incision segments
- The epithelial community is additional elaborated by lateral branches on the aspect of principal ducts
- The equine influenza viruses share ancestors with avian viruses in the very same subtype, indicating their attainable avian origin
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- The evaluation of the amino acid residues which surround in its pharmacophore binding pose signifies
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- The examination also implies that the improvement of the amount of preparedness reduces the danger of climate-related disasters
- The examination of bioenergetic function in intact cells is important because experiments with isolated mitochondria are typically constrained to a very narrow range of conditions including saturating concentrations of respiratory substrates or ADP
- The examine is additional strengthened by utilizing two distinct techniques to measure cardiac operate (echocardiography and conductance catheter) in the very same animal
- The examine was approved by the Committee on Human Investigation (CHR) of the University of California, San Francisco (UCSF) and the VA Scientific Study Workgroup of the SFVAMC
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- The exclusion criteria were impaired renal function, chronic inflammatory or auto-immune diseases, aortic or mitral valve replacement, cancer, insulin treatment
- The exercise of PDH is controlled by means of phosphorylation of 3 serine resides (232, 293, and 300)
- The exercise of recombinant enolase was assessed by measuring the transformation of NADHH to NAD as explained elsewhere
- The existence of SAR sample supported the notion they symbolize promising direct structures for the growth
- The existence of a rescuing ORF overlapping an exon junction is much from enough in get to establish a polycistronic transcript
- The existence of a rescuing ORF overlapping an exon junction is much from enough in get to recognize a polycistronic transcript
- The existing data demonstrate that reprogramming suppresses untimely senescence phenotypes in WS cells by reversing the ageing procedure and restoring telomere upkeep in excess of the prolonged expression
- The existing examine was developed to investigate the mobile/molecular foundation of the conversation numerous human tumor cells
- The existing transient is brought on by sluggish aqueous diffusion of the negatively charged BZB compound that moves more quickly
- The experiment was recurring 3 instances, and each experimental condition was assayed in replicate (n = six)
- The experiment was recurring 3 times, and every experimental problem was assayed in duplicate (n = 6)
- The experiments shown are representative of at least three others, which gave similar results
- The experiments suggest that DcuR varieties a complicated with DcuS which may be of adequate balance to accumulate DcuR at the internet site of DcuS
- The expression of AMDHD1 in the liver is inhibited by microRNA miR-122 antisense. The miR-122 makes up 70% of all microRNA in the grownup liver
- The expression of Beclin 1, the mammalian homolog of yeast autophagy gene Atg6 which is critical for induction of autophagy, is decreased in many cancer tissues and cancer cell lines
- The expression of ecto-59NT/CD73 and ADA is inversely regulated by interactions between b-catenin and TCF/LEF, resulting in a nuclear protein complex which interacts with the promoter region of these genes
- The expression of other widespread apoptosis regulators was not substantially altered
- The expression of survivin also correlates with the progression of some pathological processes of cancer, functioning through apoptotic mechanisms
- The expression profile of PMCAs depends on a fine-tuned regulation by Ca2 -dependent molecular tools such as transcription factors or alternative splicing factors
- The expressions of some genes included in glycolysis and the TCA cycle, which are shown in Figure 4 or Figure 6, are also presented
- The expressions of some genes involved in glycolysis and the TCA cycle, which are shown in Determine 4 or Figure 6, are also offered
- The extent of cell death didn't differ between handle and MRP1 overexpressing cells at a shorter duration of H2O2 remedy
- The extracted lipids were subjected to ESI-MS analysis by flow injection with liquid chromatography separation
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- The fact that muscle-specific or musclerelated miRNAs are highly enriched indicated that the data extracted from the duck breast muscle are credible
- The fact that normal cells and immortalized cells failed to respond to acid gradient indicates that the traditional ATP synthesis pathways like glycolysis
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- The final results herein validate dysregulated hemostasis at the level of the conceptus in malaria-infected mice
- The findings presented here also improve the understanding of the involvement of executioner caspases in heart development
- The findings suggest that ECFC tubule formation in the presence of FBS is, at least in part, VDR dependent
- The flow cytometry data indicated that IGF-IR is autophosphorylated in a higher percentage of brain-seeking cells, and that the mean intensity
- The fluorescein brands contained in nucleotide polymers can be found by fluorescence microscopy
- The fluorescence intensity was monitored using plate reader (PerkinElmer, Waltham, MA) at excitation and emission wavelength of 485 nm and 535 nm for monomeric form as well as 535 nm and 590 nm
- The fluorophenyl group details out from the energetic website inhibition of possibly kinase by itself
- The fluorophenyl team details out from the lively website inhibition of either kinase by itself
- The fluorophenyl team points out from the invasiveness of tumor cells than inhibition of either kinase by itself
- The fluorophenyl team points out from the invasiveness of tumor cells than inhibition of possibly kinase alone
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- The formation of a-acetolactate from pyruvate and additional conversion to
- The forward primer included a SpeI site, and the reverse primer contained three codons for glycine and six codons for histidine residues followed by a stop codon and a HindIII site
- The framework 3AE4 displays that the Br atom exhibiting a effectively described electron density has the identical orientation
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- The frequency of getting random regular r values increased or equal to the observed regular r value was taken as the P price of observing the stage of co-expression in the gene cluster with n genes
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- The frozen mobile pellets were placed in a sterile, pre-cooled (285uC) mortar and liquid N2 poured above the pellet
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- The function introduced here supports main the HCV capsid protein as a novel target for anti-HCV drug advancement with other HCV proteins
- The function of FAS in sleep regulation has not been analyzed enhanced snooze is associated to elevated FAS action
- The function of activating KIR might as a result be largely during an inflammatory response when HLA-F and MHC-I OCs are upregulated
- The function presented right here supports main the HCV capsid protein as a novel concentrate on for anti-HCV drug improvement with other HCV proteins
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- The fusion complex includes 1 t-SNARE of the syntaxin household, and two-3 cognate SNAREs
- The fusion proteins localized to the tonoplast (GFP-TIP21) or tonoplast and bulbs (rbb1-one and TIP11-YFP) in the DMSO handle as predicted (Fig 4AC)
- The gel electrophoresis was run at 100V for 2 several hours. The gel slab was then analysed with a gel imaging technique (GelDoc Reader, BioRad)
- The gels were stained with coomassie brilliant blue stain to visualize the SOD bands against the pI ladder
- The gene encodes for the a subunit of course of heterotrimeric GTP binding protein mediates signals
- The genome sequence in distinct efflux techniques are recognized to have crucial roles in multidrug resistance
- The genome sequence in specific efflux techniques are acknowledged to have essential roles in multidrug resistance
- The genomic construction of the elephant shark, noticed gar, zebrafish, medaka, fugu, and stickleback rspo3 gene was acquired utilizing the Blat plan and GENSCAN
- The genomic framework of the elephant shark, noticed gar, zebrafish, medaka, fugu, and stickleback rspo3 gene was obtained utilizing the Blat software and GENSCAN
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- The gp41 subunit is a transmembrane protein composed of an ectodomain, a single membrane-spanning domain (MSD) and a cytoplasmic domain
- The graph signifies the ration of yellow to green pixels in the plant region of the analysed prime view images from day 33
- The graph was plotted for the action of CASP3 and the statistical significance was determined by 1-way ANOVA
- The graphical illustration demonstrates the reduction of Ca2 ATPase action in hypertrophied cells whilst BDE remedy protected the enzyme from deleterious result of Ang II
- The graphs present the indicate values and standard mistakes (B) or normal deviations (D, E)
- The grass at medium N experienced reduced ShEL than that at both no N and high N, but not the grass at low N. In the same way, the grass at medium N showed increased root viability than the relaxation
- The grass at medium N had lower ShEL than that at the two no N and large N, but not the grass at minimal N. Likewise, the grass at medium N confirmed greater root viability than the rest
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- The hTERT-RPE-1 cells have been cultured in DMEM/F12 (1:one) medium, supplemented with 10% FBS and 293T cells were cultured in DMEM, supplemented with 10% FBS
- The haplotype blocks clustered in more compact blocks amid AA population in contrast to the EA populace, which showed proof of haplotypes clustering in larger blocks
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- The helix was predicted by GeneSilico metaserver. Panel B - the final 14 amino acids of a2C-AR C-terminus highlighting the arginine-prosperous stretch (underlined)
- The helix was predicted by GeneSilico metaserver. Panel B - the last fourteen amino acids of a2C-AR C-terminus highlighting the arginine-abundant extend (underlined)
- The higher expression amounts of Nectin noticed when PyLT is expressed as determined by Northern blot evaluation correlated
- The higher expression levels of Nectin observed when PyLT is expressed as decided by Northern blot investigation correlated
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- The histogram indicates the ratio of Phospho-Stat3 band intensities normalized to Stat3. Quantification of band intensity was performed with the Quantity One software
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- The hits determined in the first were examined in vitro for alteration of dependent metabolic rate
- The homology design of human transketolase was utilized to analyze the most steady contacts belonging to the dimer interface of the enzyme
- The homopiperazine ring even more improves the binding to the energetic internet site by linking
- The homopiperazine ring further boosts the binding to the lively website by linking
- The horizontal line indicates the typical of the small inhibitory focus for equally preparations which did not differ significantly
- The horizontal line suggests the common of the minimal inhibitory concentration for each preparations which did not differ considerably
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- The hypothetical protein Rv2160c, a putative tetR-family transcriptional regulator found only in pathogenic mycobacteria and positioned on the adjacent transcript
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- The identification of Not4p phospho-acceptor websites on SP/TP positions (Figure 1A) indicates that Not4p is a substrate for CDK/ cyclin kinase pairs
- The identification of a novel pterin-internet site inhibitor eight-mercaptoguanine by in silico screening
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- The identified proteins were functionally analyzed and grouped by using Uniport database
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- The images were captured utilizing a fluorescent microscope
- The immunoprecipitates were eluted with SDS sample buffer, resolved on an SDS-PAGE gel, and stained with Coomassie Brilliant Blue R-250 solution
- The immunoreactive indicators ended up quantified by densitometry and the values ended up corrected dependent on their corresponding actin ranges
- The impact of flunarizine is comparable to that of beta-blockers, but
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- The impact of sera derived from TG and WT mice on the transcription and expression of VEGF, IL-8 and MMP-nine in tumor cells was then assessed
- The implication of functional p53 as a vital determinant of drug interaction effect of the combination
- The implication of this biology to a PKC inhibitor such as enzastaurin continues to be to be investigated
- The implication of useful p53 as a essential determinant of drug conversation result of the mixture
- The importance of this amino acid residue was currently postulated who proposed to focus on it
- The important transcripts identified have been grouped in accordance to shared responses normalized to the manage
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- The increase in Necdin expression in reaction to PyLT expression was not triggered by inactivation was dependent
- The increase in Necdin expression in response to PyLT expression was not triggered by inactivation was dependent
- The increase in nNOS and iNOS expression was associated with increased NO synthesis in response to MeHg treatment
- The increased H3K4me2 enrichment indicates that the ICP4 promoter of HSV1 undergoes chromatin remodeling to a more permissive chromatin profile
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- The induction of CLN1 and CLN2 is mediated by the SCB and MCB sequences in their promoters that bind transcription components Swi4/Swi6 (SBF) and Swi4/Mbp1
- The induction of CLN1 and CLN2 is mediated by the SCB and MCB sequences in their promoters that bind transcription variables Swi4/Swi6 (SBF) and Swi4/Mbp1
- The inexperienced main was discarded, and the resuspended pellet was centrifuged again at 11,300 g for ten min
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- The infectivity indexes (rate of infected macrophages multiplied by the average number of amastigotes per macrophage) were calculated by randomly counting at least 150 macrophages per slide
- The influence did not reach importance in the greater dose of linagliptin most likely
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- The inhibitor CHIR99021 is reported to reduce expression of pro-inflammatory cytokines including TNF
- The inhibitor hence seems to be essential for numerous actions in fertilization
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- The inhibitor was used with the appropriate controls according to the kit instructions provided by the manufacturer
- The inhibitory effect of this drug is hence directly correlated to the efficacy of glutamate uptake: the a lot more economical the glutamate uptake, the more powerful the c-DGG outcome
- The inhibitory effect on mobile expansion may be correlated with cell dying
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- The inhibitory result on mobile development might be correlated with mobile demise
- The initial expression of each and every gene was altered to its opposite path (i.e. from initial up-regulation to down-regulation, or from down- to up-regulation) or to preserve the path the very same
- The initial model in the apo type of R1a was ready by removing the coordinates of cAMP from the coordinate file
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- The insert plates have been geared up by rehydrating the BD Matrigel Matrix layer with phosphate buffered saline (PBS) for two hrs at 37uC
- The insert plates have been prepared by rehydrating the BD Matrigel Matrix layer with phosphate buffered saline (PBS) for two hrs at 37uC
- The insert was taken out and media was transformed for serum-free DMEM
- The interaction in between PKC and the pseudosubstrate area for its binding
- The interaction of Necdin with p53 indicates that this delay in progress arrest is possibly associated with a immediate inhibitory
- The interaction of Necdin with p53 suggests that this hold off in development arrest is most likely connected with a direct inhibitory
- The interactions amongst the inhibitors and every of the crystal buildings ended up examined
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- The internet site densities of I-Ab monomers per RBC and TCRs per T cell have been derived using anti-FITC MHC II, anti-TCR antibodies
- The internet site densities of I-Ab monomers per RBC and TCRs per T cell were derived making use of anti-FITC MHC II, anti-TCR antibodies
