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- Most of these samples offered intermixed phylogenetic clustering independent of the geographic area however, 1 very supported cluster was composed by six of the eleven HIV-2 sequences acquired from HIV sufferers in Barlavento (Fig. four) (18:17, 22 лютого 2017)
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- A variety of stem cells, including those derived from umbilical cord blood, peripheral blood, and adult brain (18:26, 22 лютого 2017)
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- For fluorescence imaging, cells were mounted in 4% (v/v) paraformaldehyde and incubated with Sytox Inexperienced DNA stain (Molecular Probes) (18:38, 22 лютого 2017)
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- Primary human dermal fibroblasts (obtained from TCS Cellworks) were used where specified (19:05, 22 лютого 2017)
- The control cells (no HGF) have been employed as the immunofluorescent gating control (19:09, 22 лютого 2017)
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- Cell engraftment index for SB623 was assessed using monoclonal human specific antibody (HuNu) that did not cross-react with rodent proteins (19:26, 22 лютого 2017)
- PCR items have been subjected to electrophoresis bands of suitable measurement were excised from the gel, purified (Qiagen) and submitted for automated sequencing (Laboratory Providers Division, Guelph, ON) (19:27, 22 лютого 2017)
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- Fragments ended up digested with XmnI and SbfI restriction enzymes (purple boxes) and inserted into the numerous cloning web sites (MCS) of the pMAL-c5X expression vector (prime) (19:46, 22 лютого 2017)
- The University of South Florida Institutional Animal Care and Use Committee approved all procedures used in this study (19:47, 22 лютого 2017)
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