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  21. One mg of total RNA from every single sample was reverse-transcribed by 1st-strand cDNA synthesis utilizing MMLV RT (Invitrogen), in accordance to the manufacturer's recommendations
  22. One microgram of RNA from each sample was reverse transcribed using High Capacity cDNA Reverse Transcription Kit
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  25. One more gauge of TCR affinity makes use of the extent of peptide:MHC II tetramer staining detected by flow cytometry
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