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- RNA-Seq examination was limited by the amount and good quality of the cells received
- RNA oligonucleotides were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacture's Total RNA was extracted using Trizol
- RNA provisions detached from photocells 2448 after transfection
- RNA was isolated employing the Trizolbeadbeater approach
- RNA was isolated using the Trizolbeadbeater system
- RNA was isolated working with the Trizolbeadbeater process
- RNA was quantified by measuring A260 nm on the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, United states)
- RNAi treatments were compared to dsGFP-injected control mosquitoes of an anti-Plasmodium defense that involves a serine protease cascade
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