RRAD Can Provide Spanking New Life To The Old Subject- Platinum Level

That is, each constituent sequence within a PE changed proportionately with the entire set of sequences from the PE. Environmental associations among the high-frequency sequences within A-like and B��-like PEs recovered from vertical sections of cores collected at 60, 63, and 65��C were tested in a single CCA analysis, and the results for predominant PEs are displayed separately for each lineage in Figures 5A,B (see Supplementary Figure S4 for non-predominant PEs, including A�� PEs). The analyses demonstrated strong evidence of clumped distributions of the various high-frequency sequences within each predominant PE, with respect to temperature and depth (Ordtest analyses, P Veliparib variants predicted by Ecotype Simulation to belong to the same targeted putative ecotype (PE) for A-like (A) and B��-like (B)Synechococcus PEs. Small gray data points represent high-frequency ... High-frequency sequences assigned to most A-like PEs formed clusters based on their temperature and depth distributions that were unlikely to have formed by chance (significance levels for PEs were all p �� 0.025). This suggested ecological homogeneity among variants within PEs A1, A4, A7, and A14. Variants predicted to comprise PEs A6 and A12 were not significantly clustered (p = 0.141 and 0.194), but these PEs each contained three high-frequency sequences, two of which formed a significant cluster to the exclusion of the third variant, which was much more rare (Figures 6A,B and see Figure ?Figure1A1A). The most abundant PE A6 variants were distributed at depths that overlapped with variants of PE 14. The most abundant PE A12 variants were distributed near the surface of the mat at higher temperatures. All other A-like PEs exhibited unique distributions. PEA7 was found at higher temperatures than PEs A1, A4, A6, and A14, which were distributed with depth in the same order as shown in analyses of all variants within PEs (Figure ?Figure33). Conservative demarcation lumped clades that were, in some cases (e.g., PEs A6 and A12), ecologically distinct (Figure ?Figure1A1A). FIGURE 6 Canonical correspondence analyses of high-frequency psaA sequence variants predicted by Ecotype Simulation to belong to the same PE. (A,B) Clustering of predominant variants (squares) to the exclusion of the least abundant variant (diamonds) within A-like ... Each predominant B��-like PE demarcated using the conservative approach formed a cluster unlikely to have formed by chance (p �� 0.023; Figure ?Figure5B5B), again suggesting ecological homogeneity among variants within all PEs. For example, PE B��15 variants formed a tight cluster distributed toward warmer surface samples. PE B��2 formed a looser cluster distributed in cooler and deeper samples.