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  1. RNA oligonucleotides were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacture's Total RNA was extracted using Trizol
  2. RNA provisions detached from photocells 2448 after transfection
  3. RNA was isolated employing the Trizolbeadbeater approach
  4. RNA was isolated using the Trizolbeadbeater system
  5. RNA was isolated working with the Trizolbeadbeater process
  6. RNA was quantified by measuring A260 nm on the ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, United states)
  7. RNAi treatments were compared to dsGFP-injected control mosquitoes of an anti-Plasmodium defense that involves a serine protease cascade
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