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Add enough p38 MAPK apoptosis liquid nitrogen so that the level in the cryo-transfer station is high enough to just submerge the sample. Use the previously cooled screwdriver to open one of the lids and loosen the corresponding screw that is?keeping the TEM grid in place. Use the previously cooled tweezers to pick the TEM grid and place it into the TEM holder. Use the cooled hexring to fasten the TEM grid onto the TEM holder. Close the shutter of the cryo-transfer TEM holder. The sample transfer step is crucial and might be hampered by nitrogen gas reducing the visibility of the small TEM sample. Disconnect the cryo-transfer station from the pumping system and transport it near the TEM, together with the heater controller of the cryo-transfer TEM holder. Start the turbomolecular pump of the TEM to pump the backing line to the TEM airlock. Set the TEM sample stage to a tilt* of -70��. Set the shortest pumping time for the airlock (30-60 sec), with only one cycle of purging with dry nitrogen gas. Ensure that the protective shutter on the cryo-transfer TEM holder is closed. Remove the TEM holder from the cryo-transfer station and insert it into the tilted goniometer (liquid nitrogen will spill out of the TEM holder Dewar). The pumping cycle will start. Once the cycle is completed, set the goniometer to tilt back to 0�� and, at the same time, hold the TEM holder so that it does not rotate with the goniometer. Insert it fully inside the TEM. During this step, the cryo-transfer TEM holder should be connected to its heater controller to monitor the temperature. The procedure to insert the sample holder into the TEM may vary between different TEMs. It is therefore recommended to contact the TEM manufacturer to obtain the appropriate procedure. Refill the cryo-transfer TEM holder Dewar. Wait for the vacuum in the TEM to reach an acceptable level. Representative Results In this work we made use of: a dual beam FIB/SEM equipped with a nanomanipulator and a cryo-preparation chamber; a TEM with a cryo-transfer holder; a prototype cryo-transfer station. The anticontaminator (AC) blades of the cryo-preparation chamber and the tip of the nanomanipulator (NM) were modified by Gatan. With respect to a standard cryo-preparation chamber, the AC blades are larger to provide a greater heat sink for the NM tip. Moreover, the AC is fitted with clamps for connecting the Cu braids for heat exchange with the NM tip. The pneumatics of the FIB/SEM were modified to allow the NM to be and remain inserted even when the sample chamber was vented. It should be noted that the parameters used in this work are best suited for the equipment listed above; those parameters may needed to be adjusted when working with other types of equipment. To work with this protocol, the normal precautions for handling cryogenics, liquid nitrogen and vacuum systems should be followed.