A PLX3397 Research Dash Widget
Methods Nucleic acid extraction and purification: colony material from pure cultures is suspended in 100?��L molecular-grade water. For the negative isolation control (NIC), 100?��L molecular-grade water is used. DNA has been isolated from bacterial suspensions of approximately 1?��?108?cfu?mL?1 according to the protocol of the High Pure PCR Template Preparation Kit (Roche, Switzerland). 2.1 ?Polymerase chain reaction (PCR). Reagents (working concentration in brackets) X.?euvesicatoria X.?vesicatoria Final concentration X.?gardneri X.?perforans Final concentration Per reaction (��L) Per reaction (��L) Molecular-grade water* 15.75 ? 15.0 ? PCR Rxn ALOX15 reaction buffer (10�� Invitrogen) 2.5 1x 2.5 1x MgCl2 (50?mM, Invitrogen) 0.75 1.5?mM 1.5 3.0?mM dNTPs (10?mv each) 0.5 0.2?mM 0.5 0.2?mM BS-XeF (10?��M) 0.5 0.2?��M �C ? BS-XeR (10?��M) 0.5 0.2?��M �C ? BS-XvF (10?��M) 1.0 0.4?��M �C ? BS-XvR (10?��M) 1.0 0.4?��M �C ? BS-XgF (10?��M) �C ? 0.75 0.3?��M BS-XgR (10?��M) �C ? 0.75 0.3?��M Bs-XpF (10?��M) �C ? 0.75 0.3?��M Bs-XpR (10?��M) �C ? 0.75 0.3?��M BAC16S-F (10?��M) 0.15 0.06?��M 0.15 0.06?��M DNA Damage inhibitor BAC16S-R (10?��M) 0.15 0.06?��M 0.15 0.06?��M Platinum Taq (5?U?��L?1, Invitrogen) 0.2 1?U 0.2 1?U Total 23.0 ? 23.0 2.2 ?PCR cycling conditions: 2?min at 94��C, 40 cycles of 30?s at 95��C, 30?s at 64��C, 30?s at 72��C, 10?min at 72��C, and cooling at 20��C. 3. Essential procedural information When PLX3397 supplier these conditions are met: A test will be considered positive if amplicons of bp as indicated above for PIC and PAC are produced. A test will be considered negative if it produces no band or a band of a different size. Tests should be repeated if any contradictory or unclear results are obtained. 4. Performance criteria available 93%. 4.4 �Data on reproducibility: 100%. ""The sowing area of winter oilseed rape in Latvia has been rapidly increasing during the last 10?years, therefore oilseed rape diseases have become an important risk factor. The present paper reports 4-year data (2008�C2011) on different fungicide application systems (including forecasting systems) tested in field trials in Central Latvia. The development of stem canker (Leptosphaeria spp.) and white stem rot (Sclerotinia sclerotiorum) was evaluated depending on fungicide application schemes. Stem canker was found to be a widespread disease, and coexistence of both L.?maculans and L.?biglobosa was established in Latvia. There was no epidemic of white stem rot observed during the investigation period (incidence of disease was only 0.7�C4.5%). Application of fungicides significantly (P?
