Be Careful About Montelukast Sodium Difficulties And also A Way To Identify Them

It was given in Table 3. Table 3 Kinetic modeling of in vitro drug release Kinetic modeling of in-vitro drug release The kinetic study revealed that phytovesicle gel follows zero order kinetics as the regression coefficients approaches unity, indicating the drug release is independent of drug concentration. The n values from Korsmeyer Peppas model show that the drug release pattern follows mainly nonfickian diffusion mechanism, the summation of both diffusion and dissolution controlled drug release. Microbiological evaluation Microbial contamination The microbial contamination of the phytovesicle gel after 24 h was found to be 1.0 colony-forming unit (CFU) for bacteria and 1.51 CFU for fungi at temperature of 37��C and 2.19 and 1.76 CFU for bacteria and fungi at the end of 5 days. The values were within the limits. In-vitro antifungal click here activity by cup plate method The antifungal activity of the phytovesicular gel, conventional carbopol gel and marketed formulation was carried out against C. albicans. The zone of inhibition was found to be 30 �� 0.57, 24 �� 0.04 and 27 �� 0.60 respectively and shown in Figure 8. The antifungal activity of phytovesicle gel was higher than the marketed formulation (P Montelukast Sodium inhibition in mm Zone of inhibition by cup plate method Skin irritation studies The phytovesicle gel did not show any skin irritation as the irritation score was zero. Skin morphological studies Histology of pig ear skin treated with phytovesicle gel, conventional carbopol gel, water and marketed formulation EPZ-6438 mw was given in Figures ?Figures99-?-12.12. In case of carbopol gel (control) and skin treated with water it was clear from figure that stratum corneum, epidermis and dermis were closely packed. Whereas, in case of phytovesicle gel, the stratum corneum is highly disrupted and hence rapid permeation of the drug through the skin, hence easily by passing the skin barrier thereby enhanced skin permeation. Thus, the mechanism of penetration of phytovesicles can be confirmed from the histological study that phytovesicle gel undergoes enhanced permeation as it alters the lipid fluidity of the stratum corneum. Figure 9 Skin treated with phytovesicle gel Figure 12 Skin treated with marketed formulation Figure 10 Skin treated with conventional carbopol gel Figure 11 Skin treated with water Stability studies Stability studies of phytovesicular gel at room temperature (30��C �� 2��C) and at refrigerator temperature (4��C �� 2��C) were carried out for 6 months. The physical appearance does not show any significant changes compared to the freshly prepared formulation.