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Assays for chemokines and cytokines in serum The concentrations of IP-10, MIG, IL-8, MCP-1 and RANTES in serum were measured Dabigatran simultaneously using human chemokine cytometric bead array (CBA) reagent (Becton Dickinson Biosciences Pharmingen, CA). Samples were analyzed on a multifluorescence BD FACSCaliburTM flow cytometer using BD CellQuestTM software and BDTM CBA Software. The assay sensitivities of these five chemokines were 2.8, 2.5, 2.7, 1.0, and 0.2 pg/ml, respectively. The coefficients of variation of all chemokine assays were less than 10%. Statistical analysis Statistical analysis was performed using software package (Statistica, version 9.0) and data presented as mean values �� SE. Kruskal-Wallis and Mann-Whitney U tests were used for comparisons between groups. Correlations between variables were tested using Spearman's test. P-values less than 0.05 were considered statistically significant. Results Chemokines RANTES/CCL5, MIG/ CXCL9 and IP-10/CXCL10 were abundant in all the samples. Chemokines MCP-1 and IL-8 were detectable in 62.3% and 83.6% of samples, respectively. The MCP was Onalespib abundant in 27 children with asthma (61.4%, 17 stable, 10 unstable) and 11 control subjects (64%). Interleukin 8 was abundant in 39 children with asthma (88.6%, 25 stable, 14 unstable) and 12 control subjects (70.5%). The concentration of CC chemokine RANTES was significantly higher in all children with asthma as compared to their control counterparts. We observed an increased RANTES level also in patients with stable asthma when compared with children in control group. CXC chemokine MIG was significantly lower in all children with asthma. A significantly decreased level of this chemokine was seen also in children with stable asthma as compared to healthy children. IP-10 concentration was similar in the whole asthma group and healthy controls, while significantly increased levels of this chemokine in acute asthma have been observed when compared to stable asthma. For MCP-1 and IL-8, plasma concentrations were similar between all compared groups (Table 2). Table 2 Serum chemokine levels in children with asthma and healthy controls Correlation between click here chemokines and laboratory parameters Chemokine MIG concentration correlated positively with IP-10, IL-8, and CRP levels (r = 0.56, p = 0.001, r = 0.37, p = 0.01, r = 0.39, p = 0.01, respectively) and negatively with eosinophil count (r = �C0.457, p