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However, prognostic information provided by IHC expression categories and prognostic value added by using in situ hybridisation (ISH) in borderline cases remains unclear. We have assessed HER2 status in a large well-characterised breast cancer series prepared as tissue microarray (n?=?1858) using IHC (HercepTest, DakoCytomation) and chromogenic ISH (CISH; DuoCISH, DakoCytomation) in order to identify relationships with clinico-pathological variables learn more and patient outcome. None of these cases have received anti-HER2 therapy. There was excellent overall concordance between HercepTest negative (scores 0/1+) and positive (3+) with CISH positive/negative (defined as HER2/Chr17 copy number ratio of ?2; p?check details status. In the current clinical environment, cases exhibiting IHC 2+ with non-amplified HER2 gene status are not offered targeted HER2 therapy but do exhibit aggressive clinical behavioural characteristics and therefore optimal treatment strategies for these patients need to be determined. ""Nuclear poly (ADP-ribose) polymerases (PARPs) are a family of global monitor of chromatin structure and DNA damage repair. The relative expression levels of PARP1 protein in estrogen receptor negative (ER�Cve) BC remain unclear. Therefore the aim of this study was to investigate PARP1 protein expression in ER�Cve BC with relevance to molecular subtypes and disease outcome. Methods: PARP1 protein expression was assessed, using immunohistochemistry, in a well-characterised series of ER�Cve primary operable invasive BC cases (n?=?251) Alizarin with long term clinical follow-up. Results were correlated with molecular and clinicopathological parameters and patients�� outcome. Results: PARP1 nuclear expression was classified as high or low using a cut-off of 80 H-score, determined using X-Tile bio-informatics software. One hundred and thirty five (53.8%) of the informative cases were classified showing high expression. Significant positive correlation was found between PARP1 expression and BRCA1 expression (p?=?0.002) but no association was found with p53. High PARP1 expression was observed in both basal-like (HER2�C and CK5/6 and or EGFR+) (60%) and HER2+ (64%) compared with triple negative (TN) non-basal BC classes (45%).