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Take-Home Messages ??EDTA, in particular tEDTA, has been shown to have antimicrobial and antibiofilm abilities. ??EDTA is a very good potentiating and synergistic agent when used in conjunction with antimicrobials. ??The form in which sodium-based EDTA takes in solution is pH dependent. ??Gram-negative bacterial cell walls in particular are disrupted with EDTA. ??The affinity of EDTA toward metal ions (in particular divalent ions) is high leading to the breakdown of a biofilm. ??The EPS, which makes up approximately 80% of the biofilm structure, is disrupted by EDTA. Abbreviations and Acronyms EDTA ethylenediaminetetraacetic acid EPS extracellular polymeric substance LPS lipopolysaccharide MMP matrix metalloproteinase tEDTA tetrasodium EDTA Acknowledgments and Funding Sources No funding was obtained for this review article. Author Disclosure and Ghostwriting No competing financial interests exist. The content of this article was expressly written by the authors listed. No ghostwriters were used to write this article. About the Authors Simon Finnegan obtained his MCHEM in Biological Chemistry in 2010 from the University of Sheffield, United Kingdom. Since then, Simon has undertaken a DTC-TERM PhD position at the University of Sheffield, United Kingdom, designing novel silicones to be used in currently available wound dressings in collaboration with Scapa healthcare. Steven L Percival holds a PhD in medical microbiology and biofilms, a BSc in Applied Biological Sciences, Postgraduate Certificate in Education, diploma in Business Administration, an MSc in Public Health, and an MSc in Medical and Molecular Microbiology. Early in his career, Steven held R&D and commercial positions at The British Textile Technology Group Plc, followed by 6 years as a senior university lecturer in medical microbiology, and later the positions of Director of Research and Development and Chief Scientific Officer at Aseptica, Inc. and senior clinical fellowships at the Centers for Disease Control, Atlanta, and Leeds Teaching Hospitals Trust, Leeds, United Kingdom. More recently, Steven held senior R&D positions at Bristol Myers Squibb, Advanced Medical Solutions PLC. In 2011, Steven joined Scapa Healthcare PLC as Vice President of Global R&D, Healthcare. He has written over 300 scientific publications and conference abstracts on biofilms, antimicrobials, and infection control and has authored and edited seven textbooks. He also holds the position of honorary Professor in the Institute of Ageing and Chronic Disease and Surface Science Research Centre at the University of Liverpool.""MG-132 concentration microbes, in particular bacteria, are known to cause various types of infections in both humans and animals. Antibiotics are usually the choice of treatment for infections and can be used to either kill or inhibit the growth of the microbes.