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At a MGMSPI concentration above 30 mg/L, rapid photothermal heating to the photoablation limit of 50 ��C occurred within 5 min irradiated using an 808 nm laser at 6 W/cm2 (Figure S6A). DOX can be loaded into the mesopores of MGMSPI through both the adsorption of mesoporous silica and the �ШC�� stacking interaction with graphene, RSL3 cell line forming MGMSPID. Due to the large surface area and pore volume, the loading capacity and entrapment efficiency of DOX can reach up to 0.95 �� 0.08 ��g/��g (DOX/MGMS) and 43.19 �� 3.99%, respectively. MGMSPID exhibited laser-mediated and pH-dependent release behavior (Figure 2B and Figure S6C). Low pH and laser irradiation can accelerate the release of DOX, because the pH results in a decreased electrostatic interaction between DOX and mesoporous silica, and the pH/heat dissociation results in a reduced hydrophobic interaction between DOX and graphene.19 The cumulative release was less than 25% after 24 h, which achieved the sustained release properties required for a high DOX-loading capacity. These characteristics will be useful for lethal tumors and reduce unintended damage and side effects to normal cells. In order to evaluate the therapeutic effect of MGMSPID, the in vitro toxicity of different treatments was Succimer determined by confocal microscopy. MGMSPI had no obvious toxicity for glioma, whereas chemo-photothermal therapy (MGMSPID with NIR irradiation) resulted in the highest toxicity compared to single chemotherapy (MGMSPID) or photothermal therapy (MGMSPI with NIR irradiation) (Figure 3A�CD). The CCK-8 assay was used for quantitative evaluation of cell viability (Figure 3E). The IC50 of each treatment is shown in Table S1, and the combination index (CI) of different therapies was 0.4823 (selleck chemicals ligand in this study. In order to evaluate the IP-mediated targeting ability of MGMSPID, glioma cells (U251) and normal cells (1800) were treated with MGMSPID and MGMSPD. As shown in Figure 4, MGMSPID exhibited significantly higher cytotoxicity in U251 cells, but no apparent difference in 1800 cells compared to MGMSPD. This point was confirmed by cellular uptake assay (Figure S7) and in vivo fluorescent imaging (Figure S8), which showed strong cumulative signals in glioma cells after IP modification. These results indicate that MGMSPID nanomedicine has a remarkable targeting effect to glioma. To confirm the IP-mediated targeting function of MGMSPI in vivo and demonstrate the functions of magnetic targeting and MRI, T2-weighted MR images of differently treated glioma-bearing naked mice were acquired on a 3-T MR scanner (Figure 5). Before injection, no obvious contrast signals (dark) indexed as glioma were observed in any MR image.