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Initial calculations suggested that a sample size of at least 100 in each group had an 80% power to detect a difference between means of four units with a significance level (alpha) of 0.05. A reliability analysis was performed to determine the reproducibility of results obtained from the questionnaire. For internal consistency, the Cronbach's alpha coefficient for the VSQ in this sample of subjects was 0.8. All analyses were done using S-Plus (version 3.1, Statistical Sciences, Inc., Seattle, Wash). Student's independent t test was used to determine if there was a difference in mean survey score for the a priori and post 3-mercaptopyruvate sulfurtransferase hoc groupings. A test of variances was conducted in conjunction. Equal variance refers to the pooled t test and unequal variance to the Welch test, which is appropriate if the two variances are not equal. The t value used was based on the test of variance proving significant. A similar analysis was done using the one-way analysis of variance to compare the mean score for each study group (greater than two groups) with the test statistics based on the type III sum of squares. If a RGFP966 mouse statistically significant difference in the model was found, then a Tukey's Honestly Significant Difference (HSD) was used to determine which groups differed. Descriptive statistics for dichotomous and categorical data included number of observations and percentages. The Pearson's chi-square test was used for dichotomous outcomes to test for associations in the data for post-hoc groupings. Unless otherwise noted, significance was assumed when the two-tailed P value was less than 0.05. Two hundred GP and 160 MS subjects completed the questionnaire. The age distribution of the GP group was even, with no age range http://www.selleckchem.com/products/kpt-330.html dominating (Table I). As expected, the MS group was predominately in the 18-24 year age range (71%). The majority of subjects (80% GP, 73% MS groups) reported their race as Caucasian. Forty-two percent of GP reported their income in the $25,001 to $74,999 range. Sixty-four percent of all questionnaire respondents were male (70% GP, 57% MS groups). Ninety-two percent of GP completed at least high school, 39% finished or currently attended college, and 16% had graduate school experience. Nine percent of GP had seen a vascular surgeon as a patient and 20% of the GP cohort knew one personally or professionally. Among MS, 11% were acquainted with a vascular surgeon. The mean VSQ score for the GP and MS groups was 54 and 67 (P