Couple Of PD0325901 Hoaxes And Best Ways To Protect Against Them

Table ?Table11 summarizes the range of zinc concentrations measured in the four tissue types in different selleck transgenic lines for the five gene constructs as well as the non-transgenic control. All numbers in Table ?Table11 represent a mean of three individual plants. The Supplementary Table S1 contains the means and variation for each event from each line. Table 1 Zinc concentrations in plant tissues from plants grown in grow chambers. Eighteen lines consisting of six PAT:AtZIP1, five PAT:AtMTP1, and seven FMV:AtZIP1 (Figure ?Figure11), showed significantly higher zinc concentrations in their storage root parenchyma tissue compared to the non-transgenic controls (Figures 1A�CC). None of the transgenic events of FMV:AtMTP1 showed a statistically significant increase in zinc concentration in the storage root as compared to controls (Table ?Table11). Both transgenes were capable of driving increased accumulation of Zn in storage parenchyma but promoter choices were critical for the AtMTP1. AtZIP1 under control of the patatin promoter resulted in higher maximum zinc levels, reaching almost six hundred percent (Figure ?Figure1F1F) higher than the controls, compared to only a 400% increase in plants expressing AtMTP1 under the control of the same promoter DEF6 (Figure ?Figure1E1E). The highest zinc accumulation was detected in storage roots of events overexpressing AtZIP1 under control of the constitutive FMV promoter, in which concentrations reached 75 ppm, an 800�C900% increase compared to the non-transgenic controls (Figures 1B,E). No significant increase PD0325901 in zinc accumulation was detected in any of the four transgenic plants containing the two gene construct FMV:AtZIP1- PAT:AtMTP1 (Table ?Table11). FIGURE 1 Zinc concentration (A�CC) and percentage increase in zinc concentration with respect to wild type (D�CF) in 4-months-old cassava transgenic storage roots from growth chamber grown plants. Values are mean zinc content normalized for dry weight ... Transgenic plants were also analyzed for zinc concentration in their leaves, fibrous roots, and peel (Table ?Table11). Zinc levels were found to be elevated in fibrous roots of transgenic plants with either AtZIP1 or AtMTP1 compared to controls. The largest change in zinc concentrations in these tissues was approximately 3.5 times greater in plants expressing FMV:AtZIP1. Only moderate accumulation was detected in the storage root peel layer, while levels in leaf tissues were reduced by as much as 50% compared to the non-transgenic controls (Table ?Table11). Spearman correlation analysis of zinc concentrations between fibrous roots and leaves for all transgenic events was significantly negative (r = -0.63, ???p