Cultivation examination indicated that mixtures of bacterial cultures of forest soil and compost samples differed in CFU counts as effectively as composition of isolates

Leading layer of the soil is abundant in decaying natural subject and it is characterised by a large variability of microbiota. Earthworms living in this surroundings belong to the epigeic species (e.g. Dendrobaena octaedra, Eisenia andrei, Eisenia fetida, official site Lumbricus rubellus). Endogeic earthworms are identified below the topsoil. This surroundings is characterised by a reduce quantity of organic residues and by decreasing variability of microbiota. Between endogeic earthworms belong species like Aporrectodea caliginosa, Aporrectodea rosea, Octolasion lacteum. Anecic earthworms (e.g. Aporrectodea longa, Fitzingeria platyura, Lumbricus terrestris) stay in burrows in deep mineral soil layers characterised by the lowest microbial load, but appear to the surface to feed on lifeless leaves, which they drag into their burrows. Earlier we centered on a research of pattern recognition molecule CCF in earthworms belonging into these a few ecotypes. CCF of Eisenia has a broader saccharide-binding specificity in comparison with other earthworm species [eleven]. Eisenia as an epigeic earthworm needs to be resistant from a variety of microorganisms present in the prime layer of the soil. Earthworms living in the reduced soil horizons are uncovered to a weaker antigenic force and their CCF possesses a limited pattern recognition ability. Much more variable and strong binding capacity of Eisenia CCF assumes a greater resource for the recognition of possible pathogenic germs. Heterogeneity of microbiota represents a increased strain to the immune program of earthworms and hence we can hypothesize that the microbial setting can engage in a essential part for the development of protection method of earthworms. Based mostly on this assumption we centered on the comparison of protection program of two closely connected epigeic earthworms, E. andrei and E. fetida. These two earthworms share many physiological houses but their normal surroundings distinctly differs that can affect their immune technique. The taxonomy of E. andrei/E. fetida is difficult considering that the most of current literature employs indiscriminately the time period E. fetida and usually it is not distinct, which of the two species is currently being referred to. Per -Losada et al. have established these two species dependent on mitochondrial and nuclear DNA sequences utilizing conserved primers amplifying COI fragments of most species [fifteen], whilst we created and used discrimination primers distinct only for one particular species. Distinctions in COI sequences of both species are dispersed in the whole duration of acquired sequences (Figure 1), as a result we have been ready to style appropriate sets of primer pairs. The principal edge of this sort of species-specific primer pairs is the chance to speedily discriminate E. andrei and E. fetida without the prerequisite of sequencing. As described formerly, the coelomic fluid displays many biological activities involved in the innate protection of earthworms. Around forty% of the cytolytic action of CF is triggered by the sample-recognition molecule CCF [eight].