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Then, our model could be used to estimate LY2157299 the depth of sampling necessary to reduce the number of phantoms below any prescribed threshold at different activity profiles and intensity functions. Conclusion Airborne metabolically active bacteria may alter the chemistry of the atmosphere through the biogeochemical cycling of organic compounds. However, little is currently known regarding which taxa may be active and their potential functions. Our study represents the first to use both rRNA- and rDNA-based methods to identify potentially active bacteria in the atmosphere. We found that the structure and composition of the rRNA and rDNA communities differed, and the rRNA community was characterized by the presence of a few highly active taxa and many taxa with low activity levels. Taxa that were rare in the rDNA community were the most likely to be metabolically active. Consistent with our empirical data, our model results demonstrated that compositional differences between the rRNA and rDNA communities, including the observation of phantom BAY 73-4506 research buy taxa, could be attributed to random sampling and differences in the activity of taxa and their abundance in the rDNA community. Using combined rRNA/rDNA sequencing, we were able to identify potentially active taxa in the atmosphere including members of the order Rhodospirillales, specifically A. rubrifaciens. A. rubrifaciens may be well-suited for growth in the atmosphere because it has pigments that can mitigate UV damage and because it grows well on simple organic compounds commonly found in the atmosphere. We suggest future research should combine both culture-independent and culture-dependent approaches to assess the potential activity of bacteria in the atmosphere. Culture-independent approaches could be used to identify potentially active taxa and then culture-dependent methods could be used to isolate organisms and study their physiology under various conditions (possibly using aerosolization chambers as in Krumins et al., 2014).We also suggest that future studies Arginase incorporate sampling through time, particularly over diurnal and seasonal time scales, to gain a better understanding of the relative roles of different environmental variables in structuring the communities. As with any environment, understanding both the structure and function of microbial communities in the atmosphere is needed to assess their potential impact on ecosystem processes such as carbon cycling. This study opens the door for future investigations of the diversity and function of bacterial communities in the atmosphere. Author Contributions AK, BB, DJ, DL, and JG designed the experiments. AK and DL performed the experiments. DL contributed new analytic tools. AK and DL analyzed the data. AK, DL, and JG wrote the manuscript. AK, BB, JL, and DJ, JG reviewed drafts of the manuscript.