However, this additional transitional step is not necessary to account for the experimental data reported with Pgp mutants and ATP analogs

This transition is not a binding function, because there is no direct trade (1173699-31-4 affiliation or dissociation) of ATP so that the apparent ATP affinity of the occluded ATP species (Kd ), would, in truth, be the total ATP dissociation constant for the second nucleotide, as represented by the additional hints serial k1 TP, k {one ATP kt , k {t ATP ATP ATP equilibria, E ATP