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13 The chromatographic separation was achieved using a Phenomenex C-18 (4.6?��?250?mm, 5?��m) at 35?��C. The mobile phase was 0.5% AcOH in water (solvent A) and acetonitrile containing 0.5% AcOH (solvent B). The step gradient elution was started with 5% B with a flow rate of 1.0?ml/min. The percentage of B was increased Chloramben to 15% at 10?min, 85% at 45?min. At 50?min the percentage of B was changed to 95% and at 55?min this was reduced to 15%. Finally, initial conditions were reverted at 60?min. The injection volume was 20?��l. The data acquisition was performed in the range of 190�C400?nm to monitor chromatographically separated peaks. For HPLC fingerprint 254?nm was selected considering optimum signal response. The results are expressed as mean?��?SEM. Statistical analysis was done using analysis of variance (ANOVA) followed by post hoc Tukey's multiple comparison test using GraphPad PRISM version 4.01 (GraphPad software, USA). The value Hydroxychloroquine mouse of p?Bioactive Compound Library screening compared with normal control and CPAE (500?mg/kg) significantly recovered (p?