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Therefore, the artery was dissected free from its surroundings and ligated. After clamping the vessel, a plastic cuff was sleeved over both ends; the artery was everted over the cuff and ligated with an 8.0 Silk ligature. Subsequently, the caval veins were sleeved over the cuffs and ligated, thereby creating a venous interposition. After clamp removal, turbulent flow through the vein graft confirmed successful engraftment. Before injection, C1inh was dissolved in sterile NaCl 0.9% (w/v) in H2O. Mice received intravenous injections (150?��L) of the dissolved C1inh according to the following scheme: 30?min before surgery 18.5?U; t?=?3 days 12.5?U; t?=?7 days 12.5 U, t?=?14 days 12.5?U and t?=?21 days 12.5?U. Animals in the control group received intravenous injections with vehicle (sterile NaCl 0.9% (w/v) in H2O), within the same scheme. At time www.selleckchem.com/products/AZD8055.html of sacrifice, 5?min of in vivo perfusion-fixation at 100?mmHg with 4% (m/v) buffered formaldehyde was followed by harvesting of the vein graft. The vein grafts were then embedded in paraffin for (immuno)histochemical analysis. Data analysis was performed with GraphPad Ceramidase and SPSS 17.0. The data were normally distributed and to evaluate whether observed differences were significant, One-way ANOVA analysis combined with Bonferroni's multiple comparison test or the non-parametric Kruskal�CWallis test were used. A p-value (two sided) of less than 0.05 was considered to be significant. The saphenous vein graft segments of patients were perfused with autologous blood either or not supplemented with purified human C1inh (4?U/mL). The perfusion blood was analyzed for the concentration Anticancer Compound Library clinical trial of active C1inh before perfusion and at 1?h intervals until 6?h after perfusion. In the blood supplemented with C1inh, the concentration of active C1inh was 2854?��?1248?��g/mL prior to perfusion, compared to 146?��?56?��g/mL in the un-supplemented blood (p?
