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Results are shown in Table 2 in which each variable was adjusted for all other variables in the table. All variables that were LY294002 statistically significant in the bivariate analysis (Table 1) but lost their significance were removed from the model by the stepwise procedure. Results showed that patients who did not rotate their insulin-injection sites were 118 times more likely to have LH compared to those who systematically change their injection sites. Patients with HbA1c levels �� 7% were at several fold increased risk of developing LH than those having HbA1c Selleck Lapatinib 7.4 times more likely to develop LH than those using injection needle of 8-mm length were (P S6 Kinase of routinely generated data. It may also be related to the case-mix of patients regarding duration of insulin therapy and the standard of care they received, ie, the systematic rotation of insulin-injection sites and the used needle sizes. The NCDEG, where the study was conducted, is a referral center that admits patients from all over the country. However, patients may also seek treatment in the Center directly. Our patient-mix, therefore, is likely to over-represent advanced patients with longer duration of their disease who are more likely to have LH.