Just Too Occupied To Take Care Of Carboplatin?

( 42) and Muzard et?al. ( 24) for unstructured single-stranded DNA (open triangles in Fig.?4). The individual sequences of the 11-hairpin structures are listed in the Supporting Material. They share the general design 5��?anchor?(T50)?hairpin?(T30)?3��5��?anchor?(T50)?hairpin?(T30)?3��with a (T)50 linker region between the anchor and the hairpin, and another poly(T) sequence for threading. We labeled the sequences according to the content of their hairpin stem, e.g., GC12 contains the hairpin-forming sequence GCGCGCGGCCGCTTTTTTTTTTGCGGCCGCGCGC.GCGCGCGGCCGCTTTTTTTTTTGCGGCCGCGCGC.Because repetitive sequences like GCGCGC have various alternative structures, we also tested a number of hairpins with nonrepetitive click here sequences, which are denoted by HP6-8 according to the length of their stem region. To further probe our thermodynamic free DZNeP energy landscapes, we also constructed a hairpin HP8m that contains a mismatch within the stem region. The thermodynamic stabilities of the hairpins span a wide range from just 3.5?kBT for AT6 to 42.5 kBT for GC12 (we use the thermal energy scale kBT as a physical energy unit; at room temperature, 1?kcal/mol �� 1.6 kBT). We have two pairs of hairpins, which have approximately the same stability, AT6GC6 and GC6AT6 with 28 kBT, as well as AT4GC4 and GC4AT4 with a shorter stem and only a 16 kBT stability. As explained in Materials and Methods, we used?a G-quadruplex structure as anchor for the strong hairpins, whereas a strong hairpin served as anchor for the weaker hairpins. It is known that the G-quadruplex can be trapped inside the vestibule of the pore (27). To test whether this has an effect on our measurements, we used both types of anchors for the hairpin GC4AT4. The results, shown in Fig.?S2, are mutually compatible. Because Carboplatin GC4AT4 is the weakest of our hairpins with G-quadruplex anchor, this indicates that none of our measurements is affected by quadruplex-pore interactions. This is consistent with the fact that the voltage required to eject the G-quadruplex out of the pore is