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Data on age, hospital admission and outpatient attendance were included as categorical variables reflecting the underlying distribution of the data. A sample size of 400 isolates was required to estimate Histone demethylase a PVL prevalence of 10% with a 95% CI of 7�C14%. We used stata, version 10 (StataCorp, College Station, TX, USA), for all data management and analyses. Descriptive statistics were used to summarize the clinical and demographic characteristics of the patients and the microbiological characteristics of the sample. We estimated odds ratios for the association between age, gender and PVL-SA disease and examined the effect of healthcare contact, infection type, infection source and previous isolation of S.?aureus. A logistic regression model was developed to identify risk factors for PVL disease by sequentially adding in factors associated with outcome, starting with those most strongly associated, and assessing for interaction with age. Results were presented as adjusted ORs with 95% CI and p-values. Three hundred and ninety samples were available for analysis. Thirty-eight of 390 specimens (9.7%, Apoptosis inhibitor 95% CI 7.0�C13.1%) contained the genes for PVL, and 66/390 (16.9%, 95% CI, 11.8�C18.6%) were methicillin resistant. 0.8% (3/390) of S.?aureus isolates contained PVL genes and were methicillin resistant and 9.0% (35/390) contained PVL and were methicillin-sensitive. 20.8% (22/106) of isolates from skin and soft tissue infections and 10% (1/10) from pus contained the PVL genes (Table?1). PVL-SA strains were rare amongst infections that are traditionally healthcare-associated such as ulcers (0/25). Compared to individuals aged Enzalutamide and soft tissue disease and these samples were four-fold more likely to contain the PVL genes compared to all other sample types [20.8% (22/106) vs. 5.6% (16/284), OR 4.4, 95% CI 2.2�C8.9, p?