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The silver stained electrophoresis reveals several clear bands in argan oil with molecular weights from 3 to 98?kDa (Fig.?1A). This protein pattern is quite similar towards the major proteins to the one observed for argan nuts after Coomassie blue staining (Fig.?1B). Immunoblotting was carried out on argan oil extract electrophoretically transferred to PVDF membranes using 1/10 diluted serum from the patient allergic to argan oil or a control serum. Bound IgEs were detected by peroxidase-conjugated goat anti-human IgEs (KPL), using ECL Plus western blotting kit (GE Healthcare Europe GmbH, Saclay, France). Immunoblotting analysis shows IgE reactivity to one band of about 10?kDa. This band disappears after inhibition with 1mg/ml of proteins extracted from argan nuts (Fig.?1C). During the last 20?years, learn more argan oil became an expensive oil, consumption of which extended to western and Far Eastern countries because of its fatty acids profile (3). It is expected that new cases of allergy to argan oil could appear. The identified allergen is a protein of 10?kDa, persistent in oil. This protein could belong to the family of oleosins which are known to be potent allergens as described for peanut and sesame (4, 5). The ability to induce severe reaction at low doses is underlined by the systemic reaction induced by prick-test and the low reactogenic dose. It must be taken in consideration by oil producers that allergenicity of argan oil could be suppressed by step of refining (6). We thank the Dr Abdellah Harguil (Agadir, Morocco) who has kindly provided Proteasome assay us the serum of the patient allergic to argan oil studied here.""A 4-year-old boy with a history of mild eczema presented with allergic symptoms after chewing and swallowing a single macadamia nut. Minutes after swallowing this, he developed a funny taste in his mouth, swelling of his eyes, became upset and started to cry. He was taken to the local A&E department where examination confirmed wheezing. He was given treatment with antihistamines, nebulized salbutamol and prednisone. There was no choking, pallor, fainting, diarrhoea, vomiting Tolmetin or rash. After an overnight stay in hospital, he was discharged. His serum was tested using the Phadia ImmunoCAP system. Total IgE was 113?kU/l. Specific IgE to almonds, Brazil nuts, cashews, hazel nuts, peanuts and walnuts was